Introduction: Chronic otitis media(COM) is a condition in which middle ear inflammation cause changes in the tympanic membrane. These changes are tympanic membrane(TM) perforation, retraction pockets, tympanosclerotic patch, cholesteatoma,etc. Ossicles may or may not be involved. Platelet rich plasma is an autologous platelet rich concentrate containing several growth factors and cytokines. It accelerates wound healing. Myringoplasty is defined as the surgical repair of the tympanic membrane. Objective: To assess the efficacy of use of autologous platelet rich plasma in tympanic membrane perforation closure during myringoplasty. Materials And Methods: For this study 66 patients were observed during the study period. Among them 33 patients were in group 1 in which platelet rich plasma was used in myringoplasty and the remaining 33 patients were in group 2 in which platelet rich plasma was not used in myringoplasty. Post operatively patients were followed up. Results: In group 1 the tympanic membrane closure was found to be 100% with no failure. In group 2 the tympanic membrane closure was found to be 78.79% with failure seen in 21.21%. Odds ratio came to be 18.96 and the results were statistically significant. Conclusion: Platelet rich plasma helps in better tympanic membrane closure and thus improves the outcome of myringoplasty