Abstract Background: The incidence of T2DM is growing at an alarming rate worldwide and is a major global health burden. The capability of autonomic nervous system to regulate and maintain a proper homeostasis can be assessed by finding out the autonomic reactivity to a physical stressors like that of exercise or exposure to ice cold temperature. The cold Pressor test (CPT) which is considered to be a sympatho-excitatory maneuver is a simple, noninvasive and validated test of sympathetic activation. The arterial blood pressure (BP) responses to CPT could be used as indicators of global sympathetic activation, and thus of cardiac status and autonomic function in human beings. Aims: To compare the autonomic reactivity by recording the arterial Blood pressure before,during and after CPT between healthy offspring with parental history of T2DM and their counterparts. Methods: This study consists of 40 healthy adult male subjects with family history of T2DM (cases) and 40 healthy adult male subjects without family history of T2DM (controls) in the age group of 18-25 years. BP during and after CPT were compared between cases and controls. Results: Chi-square/ Fisher Exact test has been used to find the significance of study parameters on categorical scale between two or more groups. In the present study, the post CPT changes in Systolic Blood Pressure (SBP) and Diastolic Blood Pressure (DBP) did not demonstrate any significant differences. However, the graphical representation of SBP changes after CPT shows fluctuations among cases before it reaches the baseline values, whereas, in controls the decrease was at constant level throughout. Conclusion: The results suggest there was altered autonomic reactivity to physical stressor among the offspring with parental history of T2DM when compared to their counterparts and hence this points towards the fact that they are at a risk of developing future autonomic dysfunction and cardiovascular complications.