Due to the increased resistance rate of Escherichia coli and Pseudomonas aeruginosa among gram-negative bacteria, the risk factors of drug-resistant negative bacilli in patients should be evaluated in the development of initial anti-infective treatment regimen in clinical practice. In this study, multidrug-resistant bacteria accounted from the strains obtained by wound cultures, which may be related to the patients with serious illness admitted to our hospital as a tertiary care hospital and the more complex history of antibacterial drug use. This study puts in a sincere effort to find the risk of diabetic foot ulcer with multidrug-resistant organism infection.