ABSTRACT Background: The gallbladder is stimulated to contract and expel the bile in to duodenum by the hormone cholecystokinin pancreozymin (CCK) produced by the endocrine cells of the duodenal mucosa in response to food (Norman S. Williums, Bailey and Loves, 25th edition). Aims and Objectives: Study of Clinical profile and factors associated with Gall Stones at tertiary health care center. Methodology: This was a cross-sectional study in the patients of Gall bladder disease in the department of Surgery of a tertiary health care center during the year January 2016 to January 2017 so during one-year period there were 106 patients after written consent were included into study. The data was presented in the Tabular form and expressed in the percentages. Result: In our study we have seen that The majority of the patients were in the age group of 40-50 were 30.19%, followed by 50-60 -26.42%, >60 were 19.81%, 30- 40 were 14.15%, 20-30 were 6.60%, <20 were 2.83%. The majority of the patients were Female i.e. 55.66 %, followed by Male- 44.34 %. The most common clinical features were Belching in 90%, followed by Heartburn in 85%, Nausea -80%, Vomiting in 78%, Bloated feeling after meals in 74%, Intolerance to fatty or fried foods in 70%. Bitter taste in the morning in 65%, Epigastric discomfort in 59%, Pain in right shoulder in 49%, Back pain in 43%. The most common associated factors were Age >40 in 76.41%, Obese (BMI > 30) in 60%, Female -55.66 %, Family history in 50%, H/o Diabetes -49%, H/o Liver disease -43%, Non- Veg diet-40%, Diet low in fiber -39% Conclusion: It can be concluded from our study that The most common clinical features were Belching, followed by Heartburn, Nausea etc. and the most common associated factors were Age >40, Obese (BMI > 30), Female sex, Family history, H/o Diabetes, H/o Liver disease, Non- Veg dietDiet low in fiber etc.