: The nativity of Indian medicine stems from the ancient science of Ayurveda. It has recognition globally in the present era, but has deep roots since ages for its well known clinical management and preventive aspects. However, the lesser explored entity remains to be the diagnostic aspect. Just as one can say that identification of the problem is half the solution, appropriate diagnosis forms the first line of treatment. To overcome the callow and also to probe much on this, the study on the unpublished manuscript Nadi Vijnaniyam was taken up. The content of the manuscript is elaborated in twelve patalas (chapters). Each patala intends to brief about the science of Nadi and the interpretations pertaining to the knowledge of Nadi with close relation to Ayurveda. The content of the manuscript authenticates further that this will be highly contributory to the existing Ayurvedic literature. Analysis of this text brought to light, the understanding of the distinct content and few insights that are exclusively belonging to Nadi Vijnaniyam.