Background: Timolol, brimonidine and latanoprost are three different classes of drugs used in the treatment of glaucoma. These drugs act through different mechanisms to reduce the intraocular pressure (IOP). The present study aimed to evaluate the effectiveness of timolol 0.5%, brimonidine 0.2% and latanoprost 0.005% in treatment of glaucoma. Methods: This three arm parallel group randomized study was done at Narayana General Hospital & Modern Eye Care Hospital, Nellore. This study included 30 patients randomly allocated to three groups 1, 2, and 3 receiving treatments with timolol 0.5%, brimonidine 0.2% and latanoprost 0.005% respectively. Results: Total 10 patients were included in each group (1, 2, 3). The baseline characteristics were comparable between the groups. The group 1 patients showed significant decrease in IOP on day 3 of treatment, in comparison to baseline IOP. The same was observed with other groups also. In comparison of mean reduction of IOP between groups, group 3 patients showed significantly more reduction in IOP. Conclusion: Latanoprost has shown more efficacy in comparison to timolol and brimonidine in reducing the IOP.