BACKGROUND:The amniotic fluid plays an important role in fetal growth. Measurement of amniotic fluid volume can be made directly, indirectly, or estimated sonographically. Thetwo standardmethods of sonographic evaluation of amniotic fluid volume are single deepest pocket (SDP), and amniotic fluid index (AFI). We aimed to compare different methods of measuring AFI for increasing its accuracy and, subsequently neonatal outcome. METHODS: A cross-sectional study was conducted at the Obstetric and gynecological departmentfrom May 2019 to February 2020. The study included 80 pregnant women (18- 35) years old whose gestational age before 24 weeks of gestation and not hypertensive or diabetic. Women were assigned to AFI or SVDP measurement for estimation of amniotic fluid volume in lateral decubitus Position at 36 weeks, thenfollow-up measurements of AFI and SVDP were performed at 38 – 40 weeks of gestation at both postures. RESULTS: At 36 weeks of gestation, there was a statistically significant increase in AFI and VDP values on maternal change position from supine to lateral decubitus.Also, a similar significant increase in AFI and VDP values on changing maternal position at 38- 40 weeks of gestation.There was a significant increase in AFI values with the progress of gestation age at the supine position. Without significant difference at lateral decubitus position, but there was a significant increase in VDP values with the progress of gestation age at both positions.There was a significant negative correlation between AFImeasured on lateral decubitus position at 36 weeks and incidence of AF-stained meconium and need for NICU admission alsothe incidence of respiratory distress as well as there was a significant negative correlation between all VDP values and need for NICU admission and the incidence of respiratory distress. CONCLUSION:We concluded that the VDP method is the favorable method to estimate amniotic fluid volume.