Introduction: Adolescent age group is the window of opportunity to correct nutritional status of children. If we intervene correctly during this period we can prevent future consequences of nutritional deficiencies. Very few studies have been conducted in Mahbubnagar regarding adolescent anemia. Aim: To estimate prevalence of anemia and its associated factors among adolescent females from field practice areas of a Government Medical college of Mahbubnagar, Telangana. Materials and Methods: The community-based cross-sectional study was conducted in adolescent girls of rural and urban field practice area of Government Medical College, Mahbubnagar. From the computerized Health Management Information System data (HMIS), a random list of 306 adolescent girls was generated. A pre-designed and pre-tested proforma was used to obtain data regarding socio-demographic details and factors associated with anemia. Relevant clinical examination of participants were done. Blood samples were analyzed using an auto-analyser. Diagnosis of anaemia was established when hemoglobin was less than 12gm/dl.Hemoglobin level was measured for all the consented or assented participants using a digital hemoglobinometer (HemoCue201+ photometer, HemoCue AB, Angelholm, Sweden).Data analysis was done using SPSS 24.0. Association between Categorical variables were tested with Chi-square test and continuous variables independent t-test was used. The level of significance was fixed at p-value of < 0.05.