Aim: To evaluate Lipid profile of pregnant women during all the three trimesters of pregnancy. Materials and Methods: This prospective observational cross sectional study was conducted in the Department of Obstetrics and Gynaecology, Rajindra Hospital, Patiala in 1000 women attending antenatal clinic and labor room. Their lipid profile was done and proportion of dyslipidemia during pregnancy was recorded. Samples from all subjects were collected under aseptic precaution; 5 ml of non-fasting venous blood was collected in plain vacutainer from antecubital vein. After the clot retracts the sample was centrifuged at 4000 rpm for 5 min the serum separated and stored at 4°C pending assay for lipid profile. Serum TGs, TC, and HDL cholesterol was analyzed by enzymatic methods with the help of Glaxo kits on ERBA Chem-5 plus semi-auto analyzer in the department of biochemistry, GMC, Rajindra hospital Patiala