AIM: To correlate and measure Serum Phosphorus, Vitamin D3, Uric acid levels in Patients of Acute Ischemic Stroke in first 24 hours. MATERIAL & METHODS: Analytical Cross Sectional study involving data from 112 patients visiting JSS Hospital, Mysuru over a period of 18 months. 56 patients admitted to JSS Hospital with the diagnosis of acute ischemic stroke were compared with a control group comprising 56 patients. The presence of ischemic stroke was confirmed based on clinical signs, symptoms, brain CT Brain, and MRI stroke protocol. Blood samples were taken from the patients in both the group in the first 24 h of admission to measure serum phosphorus, vitamin D3, calcium, and uric acid levels. RESULTS: Totally 112 patients were registered for the study of which 56 were cases and 56 were controls. Serum phosphorus, vitamin D , serum uric acid levels of both the groups were analyzed and result was suggestive of elevated serum phosphorus and uric acid in stroke cases with significant p value (p<0.0001) whereas serum vitamin D level was low in stroke patients with significant p value (p<0.0001). Correlation of Serum Phosphorus , vitamin D and uric acid were independently associated in acuteischemic stroke . CONCLUSION: The study states that Serum phosphorus, Serum Vitamin D and Serum Uric acid were associated with the development of Ischemic stroke suggesting that inflammation and the dysfunction of the vascular endothelium could lead to stroke. By considering all these parameters in high-risk individuals developing stroke one can predict the early recognition of stroke and take necessary preventive steps and thus decreasing the morbidity and mortality of the disease. These biochemical blood parameters are easy to estimate and easy to interpret the results and cost effective, this can be used in resource poor setting situations compared to inflammatory markers such as Interleukins and cytokines.