Aim: To study the antimicrobial resistance pattern in an intensive care unitsetting of atertiary care hospital. Materials and Methods: A prospective-observational study was conducted for a period of one year from April 2018 to March 2019. A total of 2017 samples, such as urine, tracheal aspirates, pus, sputum, pleural fluid, cerebrospinal fluids, other body fluids, from the patients admitted in the ICUs were included in the study. Their culture and sensitivity testing were done as per the clinical and laboratory standard institute (CLSI) guidelines. Methicillin-resistant Staphylococcus aureus was detected by using 30 µg cefoxitin disk. Production of Extended spectrum beta-lactamase and carbapenamase was also determined.