INTRODUCTION:Needle stick injury is defined as a type of injury, caused during improper handling of hypodermic needles, blood collection needles, intravenous stiles, and needles that are used to connect parts of intravenous delivery systems. Indeed, NSIs are most preventable occupational hazards; it is also regarded as common hazards among health care individuals. AIM: To find out the prevalence of needle stick injury among different categories of health care workers. MATERIAL AND METHOD: A hospital based cross sectional study, was designed to observe the prevalence of needle stick injury among various health care workers of a tertiary level care hospital in Shalinitai Meghe Hospital and Research Centre in collaboration with Acharya Vinoba Bhave Rural Hospital Wardha. Study period was six months. RESULT AND OBSERVATION:Majority of participants were aged between 25-45 years of age, which was around (n=130, 68.8%), followed by professional above age of >45 years of age (n=32, 16.9%). On the basis of work experience of healthcare personnel; it was observed that, more than 70% of the health care personnel (n=135) had an exposure to needle stick injuries. CONCLUSION:Present study concludes that proportion of NSIs were high among HCWs, even though, having the knowledge about handling the needles and sharps. Training and management of needles and sharps must be introduced in effective way to minimize NSIs, for improving health and performance of healthcare individuals.