Hospital associated infections acquire while receiving health care. It appears 48 hours after Hospital admission or within 30 days after discharge. It is a major problem for patient’s safety and has a high impact of poor medical condition and as well as death. The most common infections are Blood, Urinary Tract, Areas of Surgery, Wound infections, Skin infections. The microorganisms which causes these infections are Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Citrobacter spp., Enterobacter spp., Proteus mirabilis, Acinetobacter baumanii, Staphylococcus aureus, CONS. The earlier study was based on use of standard specific antibiotic to test the pathogens. In this study, different antibiotics were used to test the sensitivity against these pathogens. The antibiotics were used for the study are Gentamycin, Ciprofloxacin, Amikacin, Ofloxacin, Nitrofurantoin, Norfloxacin, Penicillin, Nalidixic acid, Erythromycin, Amoxillin, Chloramphenicol, Azithromycin, Cefixime, Levofloxacin, Moxifloxacin to test the pathogens. The 398 positive cultures were taken, in which, 216 (54.27%) are urine sample, 46 (11.55%) are Pus sample, 16 are swab sample and 120 surgical site sample. The Antimicrobial Susceptibility was done by Disk Diffusion method (Kirby-Bauer Method). In this study, we have shown that CONS (95.2%) has highest antimicrobial susceptibility to Chloramphenicol