Objective: This study was conducted to evaluates the levels of pro-inflammatory cytokines and biomarkers in serum in various stages of osteoarthritis and establish their correlation with stages of Knee Osteoarthritis (OA). Methods: This case control study was conducted in the Department of Orthopaedics and Biochemistry in Dr. D.Y. Patil Medical College & Hospital, Pune. a total of 95 subjects between the age of 40 and 75 years were included out of which there were 63 cases of knee osteoarthritis, and 32 normal healthy subjects were included in control group. Pain at rest was assessed using visual analogue scale (VAS) and patients were assessed on the American College of Rheumatology (ACR) criteria to determine the severity. Serum samples were collected from all the recruited patients and serum levels of IL-1β, TNF-α, IL-6 , CTX-I and CTX-II were measured using ELISA.