Introduction: Metabolic syndrome is a group of physiological and metabolic abnormalities including obesity, abnormal glucose metabolism, dyslipidemia, and hypertension. Metabolic syndrome is known to be related with increased risk of cardiovascular disease, hypertension, and type II diabetes mellitus. There is growing evidence of close association between calcium and metabolic syndrome. This case-control study aimed to evaluate the association between serum calcium level and risk of metabolic syndrome. Objectives: To evaluate the role of serum calcium level in patients with metabolic syndrome. Methodology: We conducted an analytical case-Control study on Metabolic Syndrome patients (N=90) and age matched healthy controls (N=90). Estimation of serum calcium in healthy controls and metabolic syndrome patients done by spectrophotometric method. Results: We found that serum calcium levels were significantly decreased (1.7±5.25 Mg/dl) in patients having metabolic syndrome as compared to healthy controls healthy controls (1.9±10.05 Mg/dl). Conclusions: Low serum calcium levels have been associated with risk factors of metabolic syndrome, such as hyperglycaemia, hypertension, hypertriglyceridemia and insulin resistance. Low levels of serum calcium are widely accepted as risk factors for hypertension and cardiovascular diseases.