European Journal of Molecular & Clinical Medicine

Abstract

Abstract: Background : „Oral submucous fibrosis” (OSF) is chronic, progressive, and
irreversible disease but also as oral potentially malignant disorder with high probability of
malignant transformation. From a clinical as well as the histological point of view it
appears to be of major importance to establish and understand the molecular nature of the
mechanism of malignant transformation in OSF .So that these molecules could be targeted
to formulate a standard treatment for high risk cases of OSF patients in need for better
prognosis. Material and Methods: 25 Cases of “oral submucous fibrosis .” and 25 Cases of
oral Submucous Fibrosis with coexisting oral squamous cell carcinoma will be analyzed.4
μm thick sections of formalin fixed paraffin embedded blocks were immunohistochemical
stained with Polyclonal rabbit MT-1 MMP antibody , Monoclonal mouse TIMP-1 antibody
,Monoclonal mouse TGF-β1antibody , Monoclonal mouse α-SMA antibody in each case
Statistical Analysis: Sample size formula based on difference between two proportions:
This study uses the following formula for the sample size n: n = (Zα/2+Zβ)2 * (p1(1-
p1)+p2(1-p2)) / (p1-p2)2,
Discussion: The Incidence of malignant potential in OSF has been estimated to be 7-13%².
After years of persistent efforts and research, satisfactory and definitive treatment modality
still eludes us. These markers could be used on regular basis to assess malignant potential
of OSMF. This will help to formulate and decide the standard treatment plan with a better
regime of target drug therapy