Abstract

Introduction: Dengue viral infection is an arboviral disease which is transmitted by Aedes aegypti and Aedes albopictus. Dengue cases are now increasing a global burden especially in tropical and subtropical countries.¹ The patients with dengue fever have high levels of nonstructural protein-1 (NS1) protein in their serum after onset till <5 days.   The present study aims to establish most sensitive and reliable method for the early diagnosis of dengue infection.
Materials & Methods: Total 110 patients were screening who were having <5 days of history of dengue like fever at NIMS Medical College, NIMS University, Jaipur from June 2020 to Oct 2022.  For Dengue NS1 ICT & ELISA J. Mitra Pvt Ltd. Kits were used. Dengue RTPCR was done by TRU PCR 3B Black Bio kits as per standard protocol.⁵
Results: Out of 110, total 72 (65.45%) cases were positive (either by ICT, ELISA & RTPCR).  Among 72 dengue positive cases 48 (66.6%) were male while 24 (33.4%) were females. Male: female ratio was 2:1 observed.  55 patients were positive by Dengue NS1 ICT, 59 were positive by NS1 ELISA while 72 cases were positive by RTPCR.
Conclusion: Early detection and diagnosis are very important in the case of dengue infection as if it is not treated it may lead to many complications. RTPCR is the most sensitive and specific method for the early diagnosis of dengue. After this covid pandemic most hospitals have RTPCR lab facilities which can be utilized for dengue detection by RTPCR.