European Journal of Molecular & Clinical Medicine

Abstract

Context: Beta Thalassemia major is a genetic disease with an autosomal recessive pattern of
inheritance that occurs as a result of disorder in haemoglobin synthesis. In Beta Thalassaemia
major patients multiple blood transfusions, ineffective erythropoiesis and increased
gastrointestinal iron absorption lead to iron overload in the body. Iron overload can be
determined by Serum Ferritin measurement.
Aim and Objectives
1. To Assess the average frequency of blood transfusion in Beta Thalassemia Major patients
2. To measure Serum Ferritin level in Beta thalassemia Major patients
3. To determine the association between Serum ferritin level and age, average frequency of
blood transfusion in Beta Thalassemia Major Patients.
Methods and Material: Fifty blood samples of clinically diagnosed Beta Thalassemia major
patients were collected for estimation of Serum Ferritin levels. Serum Ferritin measurement
was performed using indirect enzyme linked immune sorbent based assay kit. Data were
analysed to determine association between Serum Ferritin and age, gender and frequency of
blood transfusion.
Statistical analysis used: Descriptive statistics, Regression, Correlation
Results: The mean Serum Ferritin level was found to be 3639.75 mg/ml. Five patients had
Serum Ferritin <1000 mg/ml, Twenty three patients had Serum Ferritin between 1000-3500
mg/ml and twenty two patients had Serum Ferritin >3500 mg/ml.
Conclusion: The study showed high levels of Serum Ferritin in Beta Thalassemia major
patients. Serum Ferritin levels should be monitored regularly to assess the status of iron
overload and to rationalize the use of chelation therapy and avoid complications related to
iron overload.