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  2. DETECTION the GENETIC EFFECTS of ANTIBIOTICS and PLANT EXTRACTS on E.COLI BACTERIAL ISOLATED FROM UTI PATIENTS USING RAPD MARKERS

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DETECTION the GENETIC EFFECTS of ANTIBIOTICS and PLANT EXTRACTS on E.COLI BACTERIAL ISOLATED FROM UTI PATIENTS USING RAPD MARKERS

    Authors

    • Fatima Mustafa Al-najar
    • Qanat Mahmood Atiyea
    • Adnan F. AL-Azzawie

    Iraq / Tikrit University / College of Science / Department of Biology

,

Document Type : Research Article

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Abstract

Background and Objective: E.coli bacteria is the common cause of the urinary tract infections as it is responsible for about 90% of cases of urinary tract infections and it is considered one of the main problems in hospital infected. So, this study aimed to assess the genetic effects of ciprofloxacin, trimethoprim, alcoholic pomegranate Granada extract and alcoholic Trigonella foenum on coliform bacteria. Materials and methods: The bacteria were isolated from urinary tract infections, after they were diagnosed using an optical microscope and conducting biochemical tests, then exposed to the antibiotic Ciprofloxacin, trimethoprim, and alcoholic extract of pomegranate Granada and Trigonellafoenum. Genomic DNA was extracted for all samples and Random amplified polymorohic DNA - Polymerase chain reaction (RAPD- PCR) marker was carried out using five randomprimers. Results: The results of RAPD-PCR profiles shown thate xposed to antibiotics (ciprofloxacin and trimethoprim) and alcoholic extracts (pomegranate Granada and Trigonellafoenum) lead to the disappearance or appearance new bands compared with non-exposed samples, and the highest rate of polymorphisim for all each treats and primers in sample 3 was 105.88% where the ratio % GTS for all treats and primers is 10% in the same sample. Conclusion: All treatments caused genetic changes in the DNA of E.coli bacteria cells especially the pomegranate Granada which gave the highest effect than the rest of the treatments, this indicates its efficiency in treating bacterial infections

Keywords

  • E.coli
  • RAPD-PCR
  • pomegranate Granada
  • Trigonellafoenum
  • Urinary tract Infection
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European Journal of Molecular & Clinical Medicine
Volume 7, Issue 9
December 2020
Page 75-87
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  • Article View: 374
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APA

Al-najar, F. M., Atiyea, Q. M., & AL-Azzawie, A. F. (2020). DETECTION the GENETIC EFFECTS of ANTIBIOTICS and PLANT EXTRACTS on E.COLI BACTERIAL ISOLATED FROM UTI PATIENTS USING RAPD MARKERS. European Journal of Molecular & Clinical Medicine, 7(9), 75-87.

MLA

Fatima Mustafa Al-najar; Qanat Mahmood Atiyea; Adnan F. AL-Azzawie. "DETECTION the GENETIC EFFECTS of ANTIBIOTICS and PLANT EXTRACTS on E.COLI BACTERIAL ISOLATED FROM UTI PATIENTS USING RAPD MARKERS". European Journal of Molecular & Clinical Medicine, 7, 9, 2020, 75-87.

HARVARD

Al-najar, F. M., Atiyea, Q. M., AL-Azzawie, A. F. (2020). 'DETECTION the GENETIC EFFECTS of ANTIBIOTICS and PLANT EXTRACTS on E.COLI BACTERIAL ISOLATED FROM UTI PATIENTS USING RAPD MARKERS', European Journal of Molecular & Clinical Medicine, 7(9), pp. 75-87.

VANCOUVER

Al-najar, F. M., Atiyea, Q. M., AL-Azzawie, A. F. DETECTION the GENETIC EFFECTS of ANTIBIOTICS and PLANT EXTRACTS on E.COLI BACTERIAL ISOLATED FROM UTI PATIENTS USING RAPD MARKERS. European Journal of Molecular & Clinical Medicine, 2020; 7(9): 75-87.

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