Online ISSN: 2515-8260

Keywords : IL-17

Immune Response Mechanism of the Gingival Epithelium in the Tissue Repair Exposed to Porphyromonasgingivalis Toxin on Topical Administration of Nigella sativa Extract

Shafira KURNIA; Jonathan STEVEN; Vincent LAKSONO

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 886-898

Background and Objectives: Periodontal disease is not the typical cause of pain experienced by sufferers. This discomfort is commonly observed in advanced stage. P. gingivalisbacteria infections, and Lipopolysaccharides (LPS) have been identified as the main virulence factors. Furthermore, numerous attempts have been exploited during treatment with unsatisfactory outcomes, including the administration of local or systemic antibiotics, periodontal curettage and flap surgery. Therefore, early prevention is one of the effective and efficient approaches, particularly involving the use of medical plants, and the natural material selected was black cumin (Nigella Sativa). The aim of this research, therefore, is to investigate the effects of Nigella sativa extract against the inflammation reaction and beta defensin-2 (BD-2) mechanism in gingival epithelium exposed to P. gingivalis LPS. Materials and Methods: This is an experimental research performed in the laboratory, using Randomized Posttest-Only Control Group Design. The animals used include white 45 Wistar rats (Rattus norvegicus) randomly allocated to 3 research groups, comprising K (LPS P. gingivalis), P1 (N. sativa extract + LPS), and P2 (LPS + N. sativa). Furthermore, each set was subsequently divided into 3 on the basis of time span, at 4, 7, and 21 days. The tissue expressions of BD-2, and MMP-8 were examined using immunohistochemical techniques. Results: This investigation showed a significant difference between K, P1, and P2 groups at any span of time. Moreover, lower MMP-8 expression were observed in the P1 and P2 groups, compared to K on day 4, 7, and 21, while the BD-2 expression was higher in P1 (31.3) and P2 (32.2) than K (19.8). Conclusion: Based on the study results, Nigella sativa was determined to be capable of decreasing MMP-8 expressions, and increase BD-2, as part of the host innate response.

Comparative Study Of Some Immunological Aspects Between Type I And Type II Diabetic Mellitus In Iraqi Patients Of Thi-Qar Province.

Hayfaa A. Thijail BSc; Hind M. Mousa PhD

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 3537-3546

This study designed to compare the vital role of the inflammatory immune responses and autoimmunity in development both types of diabetic mellitus by measurement of serum IL-17 , and Anti-Gad levels among patients groups and healthy by using ELISA kits . The study included sixty DM patients were divided into two groups : 30 patients with Type I DM ( 33.7% ) , and 30 patients with Type II DM (33.7%) . In addition to 29 healthy individuals were (32.6 %) . The results indicated an elevated mean serum level of IL-17 and Anti-Gad in both patients groups , as compared with the healthy control with significant difference . Also, the findings recorded a highly significant difference in IL-17 and Anti-Gad sera levels between both types of patient groups at (P< 0. 01) . According to the correlation results between IL-17 and Anti-Gad in patients groups , the data was demonstrated that the expression of IL-17 had a significant positive correlation with expression of Anti-Gad (P< 0.05) among Type I patients . Whereas an inverse very weak correlation was observed between IL-17 and Anti-Gad with no significant (P ˃ 0.05) in Type 2 patients . we concluded the an elevated levels of IL-17A in both types of DM may be as a prognostic factor for development them in Iraqi population. We also conclude that elevated Anti-Gad in Type II DM may be an indication of the rapid development of undiagnosed insulin deficiency in latent autoimmune diabetes in adult patients .