Keywords : bitter wormwood
European Journal of Molecular & Clinical Medicine,
2020, Volume 7, Issue 1, Pages 3336-3345
The work is devoted to the development of the technology of composition and the study of the chemical composition of the liquid extract based on bitter wormwood - Artemisia Absinitum L. grown in the territory of Uzbekistan. The addition of tansy and pumpkin seeds to the composition of wormwood makes it possible to obtain liquid dosage forms with an enhanced anthelmintic effect. The study used methods of spectrophotometry, thin layer chromatography, qualitative chemical analysis, ionization plasma analysis, etc. The liquid extract was standardized by quantitative determination of flavonoids. A number of factors have been identified that influence the optimal technology for obtaining a liquid extract from plant materials. A method has been developed for the quantitative determination of the amount of flavonoids using spectrophotometry in the wavelength range of 350-450 nm. The content of the sum of flavonoids in extracts varies in terms of a standard sample of rutin in the range from 1.2860 to 1.5871% (mg / ml). For elemental analysis, the method of inductively coupled plasma mass spectrometry was used, on the basis of which important macro- and microelements were found in the composition of the liquid extract.
The studied indicators of the microbiological purity of the extract comply with the requirements of the State Pharmacopoeia XI issue 2.