Online ISSN: 2515-8260

Keywords : E. coli


Coliphages Lytic; as a potential biological control agent against E. coli (O157:H7) in a mouse model.

Nidham M. Jamalludeen; Hekmat K. Atea; Hazim T. Thwiny

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 1306-1317

The aim of this study was to isolate and characterize the phage for use as a potential
biological control agent against Escherichia coli O157: H7. A standard strain of E coli O157:
H7 (NCTC 12900, China) was used to isolate the lytic phage from bovine faeces and raw
wastewater. The spot-assay test was used to check phage activity. Characterization of phage
isolates included phage resistance to acidity and alkalinity, electron microscopy and PCR
assay for stx1, stx2, and cI genes. Isolate phages were tested where a cocktail was
administered to treat mice that was given a single oral dose (108 CFU) of Escherichia coli
(O157: H7) (NTCC 12900). Three phages (P1, P3, P4) were successfully isolated from the
sewage samples as they showed strong lytical activity against E. coli O157: H7 (NCTC 12900).
The P1 and P4 phages had icosahedral head and a long, flexible, thin, inelastic tail with the
tail fibers while the P3 phage had a less rigid, long and relatively thick tail with the tail fibers.
All phages found are resistant to pH 4-9. The PCR results revealed the absence of genomic
coding for stx1, stx2, and cI in the isolated phages. All mice treated with the phage gave a
negative result for culture of Escherichia coli O157: H7.

Antimicrobial Activity Of Aloe Vera And Opuntia Ficus Extract Against Certain Pathogenic Bacteria

Lina Qays yaseen; Sura Hameed Nayyef; Nadia Ibraheem Salih; Mohammed Ahmed Mustafa

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 422-428

The aim of current study is detect the activity role of Aloe vera and Opuntia ficus extract against certain pathogenic bacteria and evaluation of Aloe vera and O. ficus extract against certain pathogenic bacteria inhibition in vitro. 100 samples were obtained from patients with different diseases at age 6 Mon. to 65 years from both genders. This study was carried out in Lab. of Biology Dept., Science College/Tikrit University, at the period from 1st of June 2019 to end of January 2020. The antimicrobial activity was performed with the agar well diffusion method. The results show the ability of Aloe vera for inhibition of bacteria according on concentration and the concentrations of 100 was caused in inhibitory effect on E. coli, Acinetobacte, S. aureus, S. epidermidis and S. haemolyticus isolates at diameter 13, 12, 11, 9.8 and 15 mm respectively. While, Opuntia ficus extract at 100% concentration was caused in inhibitory effect bacteria isolates at diameter 10, 9.7, 8.2, 7 and 12 mm respectively. The results also show Aloe vera was found to be able for inhibition of bacteria concentration of 40% was caused in inhibitory effect on E. coli, Acinetobacte, S. aureus, S. epidermidis and S. haemolyticus isolates at diameter 14, 12, 11, 9 and 15 mm respectively. While, Opuntia ficus extract at 40% concentration was caused in inhibitory effect bacteria isolates at diameter 11, 10, 8, 7 and 13 mm respectively, while other concentrations 10%, 20, 30% show less inhibitory effect compare with 100% and 40% concentrations

Synthesis, And Applications Of Copper Oxide Nanoparticles : An Overview

Anupam Kumar; Deepak Kumar

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 3890-3893

Nanoparticles are effective material for modern day technology. Copper oxide nanoparticles due to their vast applications prominently used in various fields. In the present review paper we have reported the synthesis of copper oxide nanoparticles by different methods. Apart for that we have discussed the various applications of the copper oxide nanoparticles in MRI, biological and solar cell applications.