European Journal of Molecular & Clinical Medicine

The extraoral dry time and the storage media used to store teeth before reimplantation have the greatest impact on the prognosis of avulsed teeth.
Aim: The purpose of this study was to evaluate and compare the efficacy of different storage media on viability of periodontal ligament cells at different time periods.
Materials and Methods: Fifty freshly extracted sound teeth with healthy PDL were selected for the present study. Teeth were divided into four groups with 10 teeth each depending on the storage media (HBSS, COCONUT WATER, ALOE VERA, MILK) used for storing freshly extracted teeth. Remaining 10 samples were divided into 2 groups with 5 teeth each to serve as control. Cell viability in each group was checked at 1hr, 2hrs, 4 hrs, 8 hrs and 24 hrs time period. The data were tabulated and subjected to statistical analysis using One way Analysis of Variance (ANOVA) with post hoc analysis (Tukey HSD) for comparison of means. The statistical software namely SPSS 21.0 was used to analysis of the data.
Results: The results indicated PDL cell viability was maximum at 1 hour time interval in all the groups and decreased over a period of time. Also, HBSS showed maximum percentage of viable PDL cells followed by Coconut water, Aloe Vera and milk at all time intervals tested.
Conclusion: It can be concluded that HBSS is the gold standard for the preservation of viable cells for a longer period followed by Coconut water and Aloe Vera.

Diabetes mellitus has been described as the most common endocrine disorder that impairs glucose homeostasis resulting in severe complications. Chronic hyperglycemia in diabetic patients is associated with long term damage, dysfunction and eventually the failure of organs, especially the eyes, kidneys, nerves, heart and blood vessels.
Currently, there is a renewed interest in the plant based medicines. One such herbal drug, Aloe Vera used for different clinical purposes claimed for its effect on blood glucose levels.
Total of 240 type 2 diabetes mellitus patients were enrolled and allocated to four groups viz. A, B, C and D. Group A and C will be given conventional anti-diabetic agents, however Group B and D in addition to anti-diabetic agent, Aloe Vera will be given as add on therapy.
While assessing the results and comparing the overall degree of glycaemia control between group A & B, a significant (p<0.004) difference with mean fasting and mean PP blood glucose (p<0.001) were observed. Similarly comparing the overall degree of glycaemia control between group C & D, a significant (p<0.004) difference with mean fasting and mean PP blood glucose (p<0.000) were observed. A significant difference between mean HbA1c recorded before and after the treatment in the groups were treated with Aloe Vera as add on therapy with conventional antidiabetic drugs.
The study concluded that the subjects who were treated with Aloe Vera as an add on therapy with conventional antidiabetic drugs has comparatively produced better clinical outcome in terms of glycaemia control and lower incidence of complications.

Herbal products have become increasingly important worldwide in medical and economic terms. Antifungal herbal antibacterial soap of Luliconazole were prepared & evaluated for dermal infection along with the addition of the oils and the extract of Azadirachtaindica, Ocimum tenuiflorum, Aloe barbadensis miller, Santalum album. The API used for the preparation of antifungal herbal antibacterial soap belongs to the antifungal class of azoles, inhibits the enzyme lanosterol demethylase, which is required for the production of ergosterol, which is a major component of the fungal cell membrane. It is mainly used in the treatment of skin infections such as athlete's foot, jock itch, and ringworm. The physicochemical parameters of formulations (Physical evaluation, pH, Foaming ability and foam stability) were determined. The results showed that the formulation have pH level nearly equal to skin pH, foaming index was excellent. The %drug release, % drug content, % solid content and microbial study was performed for API

Aim: The current examination was attempted to research the pharmacological screening
of depressant action for hydroalcoholic extract of aloe vera on mice.
Material and Methods: The hydroalcoholic concentrate of aloe vera were make the diverse
measurements type of 150mg/kg and 300mg/kg given to mice (p.o) of test gathering
Fluoxentine 20mg/kg given to mice (i.p) of standard gathering and controlled gatherings
were directed refined water as vehicle (10ml/kg). The idleness time were recorded for six
minutes in creature screening model of constrained swimming test and tail suspension test
model.
Results: In FST model of sadness, mean idleness time in seconds in Fluoxetine (20mg/kg)
treated gathering was 94.00, 92.00 and 90.00 seconds on day first, seventh and fourteenth
day. In Hydro alcoholic concentrate of Aloe Vera (150mg/kg) treated gathering was 88.00,
86.50 and 82.50 seconds on day first, seventh and fourteenth day, while in Hydro alcoholic
concentrate of Aloe Vera (300mg/kg) treated gathering was 81.00, 73.00 and 69.90 seconds
on day first, seventh and fourteenth day. In TST model of melancholy, mean fixed status
time in seconds in Fluoxetine (20mg/kg) treated gathering was 100.015, 95.50 and 90.00
seconds on day first, seventh and fourteenth day. In Hydro alcoholic concentrate of Aloe
Vera (150mg/kg) treated gathering was 96.885, 93.50 and 90.00 seconds on day first,
seventh and fourteenth day, while in Hydro alcoholic concentrate of Aloe Vera (300mg/kg)
treated gathering was 90.435, 86.00 and 80.00 seconds on day first, seventh and fourteenth
day 14.
Conclusion: The impact of Hydro alcoholic concentrate of Aloe Vera (150mg/kg and
300mg/kg) with standard medication Fluoxetine (20mg/kg) showed good upper
consequences for mice (p< 0.0005). Better impacts were seen by utilizing this medication.
So, the current examination recommends the better impact of Hydro alcoholic concentrate
of Aloe Vera about the stimulant impact in correlation with Fluoxetine

The aim of current study is detect the activity role of Aloe vera and Opuntia ficus extract against certain pathogenic bacteria and evaluation of Aloe vera and O. ficus extract against certain pathogenic bacteria inhibition in vitro. 100 samples were obtained from patients with different diseases at age 6 Mon. to 65 years from both genders. This study was carried out in Lab. of Biology Dept., Science College/Tikrit University, at the period from 1st of June 2019 to end of January 2020. The antimicrobial activity was performed with the agar well diffusion method. The results show the ability of Aloe vera for inhibition of bacteria according on concentration and the concentrations of 100 was caused in inhibitory effect on E. coli, Acinetobacte, S. aureus, S. epidermidis and S. haemolyticus isolates at diameter 13, 12, 11, 9.8 and 15 mm respectively. While, Opuntia ficus extract at 100% concentration was caused in inhibitory effect bacteria isolates at diameter 10, 9.7, 8.2, 7 and 12 mm respectively. The results also show Aloe vera was found to be able for inhibition of bacteria concentration of 40% was caused in inhibitory effect on E. coli, Acinetobacte, S. aureus, S. epidermidis and S. haemolyticus isolates at diameter 14, 12, 11, 9 and 15 mm respectively. While, Opuntia ficus extract at 40% concentration was caused in inhibitory effect bacteria isolates at diameter 11, 10, 8, 7 and 13 mm respectively, while other concentrations 10%, 20, 30% show less inhibitory effect compare with 100% and 40% concentrations

Periodontal disease is a chronic inflammatory disease that leads to the destruction of the bone support of the tooth. Its clinical manifestation varies from a simple gingivitis to a periodontitis. The main cause of this pathology is plaque buildup. Various chemical compounds have been introduced with antibacterial and anti-plaque properties. The main one is chlorhexidine gluconate. With the increased use of natural derivative compounds, interest in aloe vera (AV) has increased. AV has various effects on the human being, among which it promotes wound healing, has an anti-inflammatory effect, has an antibacterial and antiviral effect. Therefore the purpose of this review is to evaluate the antibacterial power of an aloe vera based mouthwash formulation. Several articles were considered through a systematic review of the different search engines. Three articles were examined which compared the effectiveness of an aloe vera-based mouthwash with a chlorhexidine-based mouthwash. Experiments on humans have been considered. The 3 papers showed a completely similar efficacy of chlorhexidine compared to aloe vera. In fact, studies have shown that this natural product has the same anti-plaque beneficial effects as chlorhexidine. It does not have the side effects of chlorhexidine. further clinical trials will be needed to assess whether long-term side effects of aloe vera are actually present. The studies analyzed have a short follow-up, so some long-term effects could not be assessed.