Online ISSN: 2515-8260

Keywords : Chromatography technique

The Effects of Purified Staphylococcal Enterotoxin A on Gene Expression of Nuclear Factor Кappa B (NF-КB) in Rabbits

Ayat Ali Mahmood; Ahmad Darweesh Jabar; Muthana Ibrahim Maleek

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 4319-4324

This study aimed to determine the effects of Staphylococcal enterotoxin A on NF-KB gene expression in rabbits. Firstly, SEA was isolate, purified and identified from one strain of Staph-ylococcus aureus collected from urine sample, and had identified by biochemical and molecu-lar methods. Then ,Staphylococcal enterotoxin A was purified by chromatography technique . All purified fractions of SEA was determined by ELISA kit. Finally, 5mg\ml of SEA was injected intravenously to rabbits in order to demonstrate its effects on NF-KB gene expression. At the end of this experiment, the gene expression of NF-KB increased to 9.1 in compression with control group with 1 of fold time (P = 0.045)