Online ISSN: 2515-8260

Keywords : Dengue virus

Elisa vs Rapid card : Comparitive evaluation for dengue diagnosis

Dr Pranjal Nema, Dr Anushi Hardaha, Dr Surendra Kumar Mahore, Dr Amardeep Rai

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 6, Pages 408-413

Background - Dengue diagnosis is routinely carried out by detection of dengue virus (DENV) antigen NS1 or anti-DENV IgM antibodies using enzyme-linked immunosorbent assays and rapid diagnostic tests. This study was aimed at evaluation of quality of diagnostic assays currently in use in India for the identification of DENV infection.
Study design - Retrospective Study.
Study Centre - Microbiology LaboratoryBundelkhand Medical College, Sagar, Madhya Pradesh
Study duration - June to November 2021
Methods and material- Serum samples from patients (n=1003) seeking dengue diagnosis were tested using J Mitra dengue NS1 antigen Microlisa kit andMedSourcedengue NS1rapid antigen diagnostic kits. The presence of NS1 antigen was taken as evidence for dengue-positive diagnosis.
Result – In this record review J Mitra ELISA identified 91% patients as dengue NS1 positive. J Mitra ELISA taken as reference, the Medsourcedengue NS1 test kit was found to be less sensitive (82.4%) and less specific (87.8%).
Interpretation &conclusions - These results confirmed superiority of ELISAs forNS1 antigen dengue diagnosis, and emphasized on improvement in sensitivity of RDTs.

Dengue detection and serotyping using multiplex real time polymerase chain reaction: Study from a tertiary care centre in Eastern India

Dr. Manish Ranjan, Dr. Shveta, Dr. Sakshi Kawatra, Dr. Saumya Singh, Dr. Abhishek Agrawal, Dr. Anupama Sharma

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 2, Pages 1475-1481

Introduction: Dengue is an arboviral infection transmitted through the bite of Aedes mosquitoes having four distinct serotypes DENV 1-4. Secondary infections often lead to increased disease severity. The study was carried out with an aim to analyze dominant serotype in circulation and to find an association between serotype and hematological parameters.
Material & Methods: A total of 163 Dengue Positive samples were subjected to serotype-specific Real-time Polymerase chain reaction to identify the serotype. Hematological parameters viz; WBC counts, Hematocrit value, platelets, lymphocyte % were also studied concurrently for these patients.
Results: The mean age of the participants was 27.2 years with male preponderance.NS1 antigen positive (74.8%), IgM (38%) and both NS1 & IgM positives (12.8%) were detected. The predominant serotype identified was DENV-2(48.4%), DENV-4(43.5%), and DENV-3 (7.9%). Statistically significant difference in platelet counts was observed in DENV-2 & DENV-4.White blood cell counts showed statistically significant differences within DENV-2 and DENV-3 serotypes.
Conclusion: Cocirculation of multiple serotypes of DENV was observed in our study. The application of molecular testing will increase DENV diagnostics, especially in secondary infections which carry more chances of adverse outcomes. We recommend the inclusion of serotype testing in the Dengue diagnostic algorithm to be able to reduce morbidity and mortality.


Raja kumar; Jothi Priya; V. Vishnu Priya; Lakshminarayanan Arivarasu

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 2307-2321

Dengue is especially affecting the tropic and subtropic regions of the planet . It's mainly caused by Dengue, a member of Family Flaviviridae. Four serotypes of Dengue are DENV1, DENV2, DENV3 and DENV4. 67,000 people are diagnosed by Dengue and it's been reported by NVBPCP. The aim of this study is to make awareness on risk factors and complications of Dengue virus among college students .The Survey on awareness about the danger factor and complications of Dengue virus among college students was conducted employing a google form link. We've distributed our survey among 100 college students. Then , we used a sampling method. Also, we've used statistical software as SPSS for our data. 59.05 % agreed that they're conscious of Dengue virus, 40.95% are still not aware . 58.10 % agreed that it had been transmitted by files, 27.62 % responded thanks to mosquitoes, 11.43 % responded thanks to Aedes mosquito, 2.86 % responded and still don’ t know. Awareness camps , social media , workshops , seminars could also be conducted to make awareness of risk factors and complications of Dengue virus among college students.