Online ISSN: 2515-8260

Keywords : intraoperative diagnosis

Primary Central Nervous System Lymphoma: A Clinicopathological and Cytomorpholgical Study from a Tertiary Care Centre

Swapna Devi, Suneeta Meena, Pramod Kumar Meena, Vedita Singh, Anushka Singh

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 10733-10738

Aim: To analyze the clinicopathological and immunohistochemical features of primary central
nervous system lymphoma (PCNSL) cases occurring in Indian patients and also study the utility
of the crush smear preparation in intraoperative diagnosis.
Material & Methods: 40 cases of PCNSL diagnosed in the Department of Histopathology, were
included in this study. Haematoxylin and Eosin (H and E) stained slides and
immunohistochemistry slides (which included a minimum of Leucocyte common antigen (LCA),
CD20 and CD79aof all these cases were retrieved.
Results: The mean age of the patients was 51.4 years and the median was 50 years (range 30-72
years). The male: female ratio in our study is 1:1. Of the 40 cases, one was a known case of
sarcoidosis (in remission). The only T-cell lymphoma case in our series had a prior history of
treatment for ulcerative colitis (in remission).
Conclusion: Our study shows that PCNSL is seen predominantly in immunocompetent patients
in India. The age of presentation is relatively young. Our study also stresses the utility of crush
smear preparation in establishing an intraoperative diagnosis.

“Assessment of lymph node status in cases of metastatic malignancy by frozen section and imprint cytology”

Dr Miheer Milind Jagtap; Dr Samarth Shukla; Dr Sunita , Vagha; Dr Ankita Tamhane; Dr Sourya Acharya; Dr. Miheer Milind , Jagtap

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2557-2551

Abstract: Introduction: Dissemination of cancers most commonly occurs by the lymphatic
route and is generally favoured by carcinomas. The best achievable goal of any surgical
procedure is removal of all the affected tissue and leave behind healthy tissue which is
entirely free of any malignant cells. Though histopathological examination is gold
standard, it is time consuming and cannot be implemented as an intraoperative diagnostic
tool. The present study utilises frozen section analysis and touch imprint cytology as
intraoperative tools and analyses their diagnostic accuracy to detect lymph node metastasis
in comparison with routine histopathology in epithelial malignancies.
Aim: To evaluate the efficacy of intraoperative diagnosis by touch imprint cytology and
frozen section analysis for the assessment of metastatic lymph node deposits.
Materials and methods: Total 76 cases of primary malignancy with suspicious metastatic
lymph nodes were investigated. Metastatic nodes were subjected to frozen section and 
touch imprint cytology and these findings were compared with routine histopathology and
the sensitivity, specificity, positive predictive value, negative predictive value and accuracy
were calculated.
Results: The sensitivity, specificity, positive predictive value, negative predictive value and
diagnostic accuracy of frozen section was found to be 97.30%, 100%, 100%, 97.5% and
98.68%. The values of the same parameters for imprint cytology was found to be 75.68%,
100%, 100%, 81.25% and 67% respectively.
Conclusions: Frozen section analysis proved superior to imprint cytology in detecting
lymph node metastasis intraoperatively. Apart from detecting the presence of metastasis,
frozen section is able to provide details regarding micro-metastasis, macro-metastasis and
perinodal fat invasion. This study predominantly evaluated epithelial malignancies and
thus proves the utility of these two intraoperative modalities in them. It also opens new
avenues for research pertaining to the utility of these modalities in various malignant
mesenchymal tumours.