Online ISSN: 2515-8260

Keywords : methotrexate

The Histological effects of Methotrexate on the lungs of albino rats: An experimental study

Shatha Th. Ahmed; Ziyad Ahmed Abed; Eman ghanim sheet AL-hyali

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 1, Pages 1289-1296

Introduction: Despite its potentially pulmonary complications, Methotrexate (MTX) is widely utilized in the treatment of arthritis. Several studies reported development of various forms of lung disease in patients treated with MTX. The aim of this study is to investigate the adverse effects of MTX on the lung structure and the likelihood of recovery following MTX withdrawal. Materials and Methods: A total of 30 albino rats were studied in 3 different groups of ten rats. Group I was considered as control whereas group II (MTX treated group), received 5 mg/ kg subcutaneous MTX (SC) for 5 days. Lastly, group III (withdrawal group) received similar doses of MTX for the same duration but sacrificed 5 days after MTX withdrawal for lung dissection and histological examination. Results: The lungs of MTX treated rats demonstrated a significant increase in the inter-alveolar thickness with concomitant mononuclear cell infiltration linked with alveolar collapse and damage. After MTX withdrawal, the changes in pulmonary structure exhibited partial recovery. This in turns suggests that MTX-induced lung toxicity was to some extent reversible. Conclusion: MTX could result in pulmonary inflammation and alveoli damages, therefore, this study recommends regular examinations for MTX patients designed for early detection of interstitial pneumonitis.


Sevara Mukhammadieva; Elnora Djuraeva; Nargiza Abduazizova

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2439-2449

patients under 30. The control group consisted of 110 healthy middle-aged
individuals. In the surveyed population, those with 1 to 5 years of age were the largest group
(38.3%), followed by those with 5 to 10 years of age (34%). In 66.3% of the patients of RA MT
was used as a base preparation. The average dose (median) of MT over the study period was
10 mg per week. 80% of patients took prednisolone in an average dose of 10 mg/day. The
content of lipids in venous blood was determined by photocolometry on Vitros SYSTEM
Chemistry DT 60 biochemical analyzer (Austria). Results: RA patients who took MT showed a
significant increase in the level of triglycerides (TG). Also, it is characterised by an increase
in TG levels and a decrease in the concentration of high-density lipoprotein cholesterol
(HDL) and an increase in the atherogenicity factor. A more pronounced decrease in HDL
levels. Significantly high indicators of the atherogenicity coefficient, which to a lesser extent
depended on the duration of MT use. Low-density lipoprotein cholesterol (LDL) and
triglyceridemia are also proved to be serious risk factors for atherosclerosis and
cardiovascular heart diseases (CHD). Also according to the results of the research, special
attention is paid to the use of hypolipidemic drugs, which is promising in improving the
prognosis and reducing cardiovascular injuries in RA patients. In the group of patients who
received additional lipidex SR, there was a decrease in the level of OHS by 17.1%, an
important shift was observed on the part of TG, this indicator decreased by 29.7% and
practically did not differ from the control indicators. HDL cholesterol in the SR lipidex group
increased significantly by 37.7%. These fibrates have a stimulating effect on all components of