European Journal of Molecular & Clinical Medicine

Introduction: Microbes elimination in the root canal system is endodontic therapy's prime goal. It is advised to utilise intracanal medicaments between the appointments to diminish bacterial counts. This study was conducted to investigate the effect of herbal solutions on antimicrobial efficacy of triple Antibiotics when used as delivery vehicle for intracanal medicament.
Methodology: 160 extracted single-rooted teeth were manually instrumented. After contaminating the canals with E. Faecalis and C. albicans, the specimens were divided randomly into 4 equal groups (n \20) for each C. albicans and Faecalis according to the intra-canal medications used: Group I: Clove Oil, Group II: Neem oil, Group III: Cinnamon oil, Group IV: Saline, All the three Groups were mixed with triple Antibiotics Ciprofloxacin, Metronidazole and Doxycycline. Microbial samples were obtained from the root canals after 24 hours, and cultures were determined after 24 hours of incubation. The Colony Forming unit was analysed with Kruskal- Wallis, Dunn's, and Chi-Square tests to find significant differences among different groups.
Results: The number of colony-forming units was significantly lower in all experimental groups compared to the control group in both agars, the highest mean rank for colony count in E. Faecalis was 34.1 and in Candida Albicans was 29.30. Neem oil had better antimicrobial efficacy compared to other medications when it was mixed with Triple antibiotics.
Conclusions: The study suggests that Neem oil increased the efficacy of triple antibiotics to eliminate the two different tested microorganisms at the root canal lumen. C.albicans was the most sensitive tested microorganism to the whole tested medications

Aim: To analyse the clinical profile of otomycosis.
Materials and method: The data of this prospective study was collected from patients attending ENT Outpatient Department at tertiary health care centre over a period of two years from JUNE 2021 to October 2022. The study population included patients attending the ENT OPD who gave history of discharge, pain, pruritis and reduced hearing. Over a period of two years, 50 patients were studied. All swabs taken were subjected to microscopic examination by 10% KOH and those positive for fungal elements were inoculated over saborauds dextrose agar. No transport media was used as swabs were immediately inoculated over Saborauds dextrose agar media for 48-72 hours at 37 degree C. The growth of the fungus was studied using gross morphology. Fungus was removed from patients ears once a week for three to four weeks via aural toileting, which included dry mopping, suction clearance, aural syringing, and forceps. The eardrops clotrimazole (4 drops 8 times a day for 4 weeks) were used regardless of the results of the culture.
Results: Otomycosis is more common in the monsoon season. Males are twice as likely to be impacted as females. The most common risk factor is swimming. Aspergillus niger, Aspergillus fumigatus, and Candida albicans are all susceptible to being killed by using Clotrimazole ear drops.
Conclusion: The widespread dissemination of spores of fungal species including Aspergillus Niger, Aspergillus fumigatus, and Candida albicans explains why they are so frequently recovered from Otomycosis. Otomycosis recurs frequently, and systemic disease is a major contributor.

Tridaxprocumbens and Azaridactaindica traditional medicinal plant life which have antifungal hobby in opposition to Candida albicans, a combination of these  plant life have now not been known for its pastime against this fungus. The reason of this research was to formulate topical gel, a combination of Tridaxprocumbens and Azaridactaindicta which has antifungal hobby in opposition to Candida albicans. The antifungal pastime take a look at of T. Procumbens and A. Indica the use of agar well diffusion approach turned into carried out. Thereafter, a topical gel formulation was prepared using Sodium carboxymethyl cellulose as a gelling agent of concentration 1; 1.Five and a couple of%. Test parameters for topical gel includes organoleptic, pH, extrudability, spreadability, diffusion, and stability take a look at.

Infections produced by yeast of the genus Candida are the most frequent fungal
infections, with Candida albicans being the most prevalent isolated species. The quick
identification of this yeast is mostly based on the formation of germ tubes in human or
animal serum. This study details the utilisation of seven different liquid media for germ
tube development at 2, 2.5, 3, and 4 hours. We looked at 193 yeasts for germ tube
formation, including 157 (81.3 percent) C. albicans. At 2 hours, C. albicans germ tube
development was most prevalent in human serum (98%), followed by brain heart
infusion broth (84%), and tryptic soy broth (65.6%).Sensitivity, specificity, positive
predictive value (PPV), and negative predictive value (NPV) for human serum germ
tube formation at 2 h were 98 percent, 100 percent, 100 percent, and 92.3 percent,
respectively. An incubation period of more than 2 hours improves sensitivity but
decreases specificity, as well as the PPV and NPV of the germ tube test in all tested sera
(GTT). In conclusion, with a 2-hour incubation period, human serum was shown to be
the most acceptable medium for GTT.

Aim: Insilico Interaction of Bioactive Compounds from Clove againstOral Candida albicans biofilm drug targets. Materials and methods: All the 3D models were obtained from PubMed and the final analysis was produced by two systems, mainly chemsketch and GOLD protein-ligand docking. Results : Here in this study we have tried to find the best compatible bio active compound of clove towards the target. Out of 5 Bioactive compounds from clove Eugenol acetate O4,O3 showed the highest docking score having h bond score to be 2.888(O4) , 2.653(O3). Conclusion: In most of the cases the docking the H bond value must be considered must be considered because the hydrogen bonds are stronger than van-der-walls bond and weaker than covalent bond as H bonds have the ability to create a bond or break a bond easily in this study we r trying to read the compatibility between the bioactive compounds and the target compound in this case its Candida albicans biofilm. Further the the research can be extended to wet lab work for further details.

Denture Stomatitis( DS) is one of the common denture related complications in patients wearing dentures after years of denture use. There have been various studies on its prevalence despite the fact that its exact etiology is still unknown. Removable dentures have served as an important prosthesis in dentistry, whereby it helps in regaining the phonetics and masticatory functions as well as esthetics in edentulous patients. The aim of this study was to determine the prevalence and risk factors that can cause denture stomatitis in denture wearers. This study was designed as a retrospective clinical study. All the patients with removable dentures (Complete dentures , Removable Partial dentures , Cast partial denture ) were taken into account and the data was retrieved. The data was analysed and results were recorded.Out of the 1415 subjects wearing dentures (complete or partial ) , 80 were found to have denture stomatitis in which 57.4% were women and 42.6% were men. The majority of the DS patients were in the age group range of 51-80 years. Temporary partial dentures (50%) were the most prevalent type of prosthesis in patients with denture stomatitis. When the medical history was reviewed most of the patients did not have any underlying systemic diseases(61.1%) did not have any medical condition, (18.5%) were diabetic, (11.1 %) were hypertensive. When all the diabetic patients(2577) were evaluated , 9.32% had denture stomatitis .In the study, it was observed that denture stomatitis was prevalent in elderly patients with slight female predilection. Diabetes was the most prevalent systemic illness associated with denture stomatitis