Online ISSN: 2515-8260

Keywords : Dengue RT-PCR

Molecular Characterization & Hematological Correlation Of Dengue Virus Among The Patients Attending Tertiary Care Centre, Jaipur, Rajasthan

Dr. Aditya Mishra, Dr. Ajay Kumar Gupta, Dr. Anshu SS Kotia, Dr. Shivani Shukla, Dr. Shivangi Shukla .

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 8, Pages 1868-1873

Introduction: Dengue is arboviral diseases spread by the bite of infected Aedes mosquito carrying these viruses. Every year, thousands of individuals are affected and contribute to the burden of health care. However, there was little information on serotypes of dengue virus circulating in this part of the country. This study was carried out molecular characterization of Dengue virus isolated from patients in our area and study correlation of prevalent serotype with severity of disease along with the hematological parameters.
Materials& Methods: 1192 dengue like illness patient samples were collected from 2018 -2020 at JNUIMSRC, Jaipur. RNA extraction was done by Qiagen viral RNA mini kit & followed byRTPCR by using type specific primers & probes were used for Dengue RT PCR.
Results: A total of 1192 dengue like illness cases who had less than <14 days of fever, blood samples were collected from various departments of JNUIMSRC, Jaipur.  Out to 1192 cases 585 (49.07%) where Dengue was found. In Dengue Majority of the patients 161 males (27.52%) & 73(12.47%) females belonged to the 21-30 yrs of age group. Out of 585 cases total 108 (18.46%) were DEN I, 163 (27.86%) DEN II, 218 (37.26%) DEN III and 74 (12.64%) DEN IV and along with this 24-mix serotype infection was found.
Conclusion: Dengue remains to be an important health problem in India and across the globe.  There is neither any specific treatment nor any effective vaccine for dengue so far available in India. So, the proper diagnosis is the first requirement for the management of the disease. DHF &DSS are life threatening. Molecular method is much needed as it hardly takes 4 hrs. To produce accurate result