Online ISSN: 2515-8260

Keywords : Bcl-xl��� p53

In vitro examination of the role of exercise-induced serotonin replication in the prevention and advancement of liver cancer via the intrinsic apoptotic signalling pathway.

Ashfaaq Ahmed; Lavanya Prathap; Selvaraj Jayaraman; Preetha. S

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 8, Pages 560-568

Background: Serotonin is an important mediator of variety of physiological processess including fibrogenesis,steatosis,cell proliferation. It functions as a neurotransmitter and vasoconstrictor. In this study we explained the influence of physical exercise which induces serotonin in modulating liver cancer and its functions as a neurotransmitter that inhibits the growth of tumors in a concentration dependent. Hence they are less likely to develop liver cancer through an intrinsic apoptotic signaling pathway through three  genes ( Bcl-xl, Bcl-2, p53).
Aim: To analyze Influence of exercise induced serotonin replica in prevention and progression of liver cancer through intrinsic apoptotic signaling pathway.
Materials and methods:
Human liver cancer cell line (HEPG2) was brought from NCCS, Pune, India. Cell viability test for seratonin and Gene expression analysis were carried out for The p53, Bcl-xl, Bcl-2 mRNA gene expression using MTT and PCR respectively. The results were analyzed using appropriate statistical tools.
Results: The p53 mRNA gene expression is increased on induction of 2mM, 4mM dosage of serotonin. Bcl-xl, Bcl-2 and % of cell viability decreases on induction of serotonin. Hence the compound has anti - cancer activity.
Conclusion: The results of the study concluded that people who exercise regularly have endogenous secretion of serotonin that inhibits tumor growth through modulating apoptotic and signaling pathways and hence they are less likely to develop liver cancer.