Online ISSN: 2515-8260

Keywords : Carbapenemase

Multidrug-Resistant Acinetobacter: Detection of ESBL and MBL at a Tertiary Care Hospital in Bihar

Kumari Ritu, Praveen Kumar, Pratulya Nandan, Sushma Kumari .

European Journal of Molecular & Clinical Medicine, 2023, Volume 10, Issue 3, Pages 305-311

Background:- Ability to develop multiple drugs resistance and biofilm formation have made Acinetobacter species an important hospital-acquired pathogen and a challenge to their effective management.
Objective:- Through this study we can isolate different Acinetobacter sps. and study their antimicrobial susceptibility patterns. Isolated resistant Acinetobacter was further analyzed for the detection of Extended-spectrum β-lactamases (ESBLs), Metallo β-lactamases (MBLs), Carbapenemase production.
Materials and Methods:- Various clinical specimens which were submitted to the Department of Microbiology, Patna Medical College & Hospital, Patna, Bihar were studied for antibiot ic susceptibility testing, detection of ESBL and MBL production by standard microbiologic a l methods.
Results:- The pre-dominant Acinetobacter species isolated was A. calcoaceticus-bauma nnii Complex (Acb complex) 167 (52.1). Among those, all A. species 127 (44.7%) were multidr ug resistant (MDR). In which 12 (4.22%) were ESBL producers and 36 (12.8%) Carbapenemases producers. The majority of A. species were resistant to cefotaxime 72.6% and cefepime 78.4%. Conclusion:- Drug-resistant Acinetobacter formed a substantial proportion of this hospital’s samples. This situation warranted stringent surveillance and adherence to infection prevention and control practices.

Detection of blaOXA-23geneamong carbapenemresistantAcinetobacter species isolated from various clinical samples

Md. Mustafa Sofiur Rahman, Dr. Ramanath Karicheri, Surendra Prasad Chauhan

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 9803-9810

Invasive infections due to Acinetobacter baumannii are among the leading
nosocomial infections in patients hospitalized in the intensive care unit (ICU). An increase in
multidrug-resistant has been observed in recent years. A hospital-based prospective study was
carried out on 287 laboratory-confirmed Acinetobacter species from various clinical samples
out of which 232 (81%) were found to be Acinetobacter baumannii and other species of
Acinetobacter were 55 (19%). Most of the isolates were obtained from pus samples (30.31%)
followed by Urine (25.43%), ET Tip (14.28%) ET Aspirate (12.19%) blood (4.18%). The
number of Isolates was more in IPD (91.99%) and less in OPD (8.01%). Among IPD
patients, the highest number of isolates were obtained from ICU (52.65%), surgery (21.97%),
and Obstetrics and gynecology (13.63%). Isolates showed the highest resistance towards
cephalosporins Ampicillin (89%) followed by Cefotaxime (87%), Ceftazidime (85%), and
ceftriaxone (84%). Among the carbapenem group, Meropenem was found more resistant in
comparison to Imipenem with a resistance rate of 61% and 56% respectively. Colistin was
found to be the most effective drug. Carbapenem resistance among other species of
Acinetobacter was 172 (61.82%) by the Modified carbapenem inactivation method (mCIM).
Out of 172 carbapenem-resistant isolates screened, 144 (83.72%) gave MHT positive that
confirms the production of carbapenemase by the isolates. A total of 140 (97.23%) out of
144 MHT positive isolates showed the presence of the target gene i.e., blaOXA-23.