Keywords : Cyclin D1
A Study of Cyclin D1 Gene Numerical Aberrations in Blood Samples of Oral Squamous Cell Carcinoma Patients of North India
European Journal of Molecular & Clinical Medicine,
2022, Volume 9, Issue 3, Pages 187-195
Introduction: The present study aims to detect Cyclin D1 gene numerical aberrations in blood samples of OSCCs patients (50) and controls (30) by using fluorescence in situ hybridization technique (FISH) along with risk factors.
Materials and Methods: Present study is an observational, retrospective Case control study comprising samples from North Indian population. Peripheral blood samples were collected randomly from histological confirmed 50 OSCC patients from outpatient department (OPD) of Oncology and ENT of regional tertiary care hospital, and also from 30 healthy controls of 25 years or above age. FISH interphase technique was used to detect the numerical aberrations of Cyclin D1 using the Vysis protocol (Vysis CCND1 CEP11 FISH Probe Kit, Abbott Molecular Inc. Des Plaines IL 60018 USA). Mean and SD were calculated. Pearson Chi-square and the 2-tailed Fisher's exact test (FET) were used for comparison of parameters association among themselves (P value <0.05).
Results: Cyclin D1 gene numerical aberrations were absent in controls; where as 5 (6.3%) cases were positive in OSCC group. Among positive cases low and high level amplification were found in 3 (3.8%) and 2 (2.5%) cases respectively. Other than all factors measured Cyclin D1 gene aberrations showed a significant association only with lymph node metastasis (P=0.004) and stage of Carcinoma (P=0.001) respectively.
Conclusions: The present study suggests the prognostic importance of blood as a medium for assessing Cyclin D1aberrations in advance stage OSCC patients.