European Journal of Molecular & Clinical Medicine

Introduction: Dengue viral infection is an arboviral disease which is transmitted by Aedes aegypti and Aedes albopictus. Dengue cases are now increasing a global burden especially in tropical and subtropical countries.¹ The patients with dengue fever have high levels of nonstructural protein-1 (NS1) protein in their serum after onset till <5 days.   The present study aims to establish most sensitive and reliable method for the early diagnosis of dengue infection.
Materials & Methods: Total 110 patients were screening who were having <5 days of history of dengue like fever at NIMS Medical College, NIMS University, Jaipur from June 2020 to Oct 2022.  For Dengue NS1 ICT & ELISA J. Mitra Pvt Ltd. Kits were used. Dengue RTPCR was done by TRU PCR 3B Black Bio kits as per standard protocol.⁵
Results: Out of 110, total 72 (65.45%) cases were positive (either by ICT, ELISA & RTPCR).  Among 72 dengue positive cases 48 (66.6%) were male while 24 (33.4%) were females. Male: female ratio was 2:1 observed.  55 patients were positive by Dengue NS1 ICT, 59 were positive by NS1 ELISA while 72 cases were positive by RTPCR.
Conclusion: Early detection and diagnosis are very important in the case of dengue infection as if it is not treated it may lead to many complications. RTPCR is the most sensitive and specific method for the early diagnosis of dengue. After this covid pandemic most hospitals have RTPCR lab facilities which can be utilized for dengue detection by RTPCR.

Background: The demographic pattern and the trend of disease (dengue fever) are
largely changing everyyear through the past decade.Early recognition and prompt
initiation of appropriate management is vital. The aim of this study to correlate the
clinical profile with laboratory investigations and radiological findings in dengue fever
at tertiary care center.
Materials& Methods: A cross sectional study done on 50 children less than 12 years of
age with clinical signs and symptoms of Dengue- any acute febrile illness in department
of Pediatrics, RUHS College of Medical Sciences, Jaipur, Rajasthan, India during one
year period. For all suspected dengue fever, the IgM ELISA qualitative test was done at
our hospital. Children positive for IgM dengue were taken up for study and followed up
for clinical profile.Labinvestigations carried out in these patients include CBC,
Haemoglobin, renal functiontest, Liver function test and Dengue IgM serology. Chest X
ray was taken todemonstrate pleural effusion. Children positive for IgM were followed
up for the clinicalprofile and outcome.
Results: Our study showed that the larger number of cases was in 6 to 12 years.).Males
were affected slightly more than females in total and also insubgroupsexceptin DHF
III.Itwas not significant (P>0.05).The mean duration of fever was 6.23 days.Pleural
effusion was seen 6 patients, of which more cases in dengue feverwith warning sign.
Abdominal tenderness was seen in 46% of cases, highest in denguefever with warning
signs and lowest in mild dengue which is statistically significant.Ascites was seen in 20
% of cases, highest in dengue fever with warning signs andDHF IV. Hepatomegaly was
seen in 30%, highest in DHF IV and lowest in mild dengue which is statistically
significant. The splenomegaly was seen in 4% of cases. AST was elevated in 36% of
cases. More in DHFIII & DHFIV cases, but it is not statistically significant. ALT was
increased in 30% of cases. More elevated cases are in DHFIII which was also not
significant.

Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the dengue virus which is transmitted by the Aedes sp Mosquito. The Incident Rate (IR) in the Rowosari Health Center area in 2018 is still high, which is 98/100,000 residents. This study purpose to analyze the correlation between the rate of larvae and several environmental factors with the incidence of dengue in the work area of Rowosari Health Center, Semarang City. The research is a descriptive study with a cross-sectional approach, carried out by the analytic survey method. The sample in this study was houses located in 5 urban villages in the Rowosari health center area chosen by purposive sampling method. The results of this study indicate that the Rowosari Health Center work area has a House Index value (17.5%), Container Index (5.7%), Breteau Index (19.5%) with density figure 2-5. The results of the Pearson correlation test showed no significant correlation between House Index (p = 0.126), Container Index (p = 0.494), Breteau Index (p = 0.094), population density (p = 0.919), temperature (p = 0.384), humidity (p = 0.229) with the incidence of DHF in the Rowosari health center work area. It is recommended that the community routinely clean containers as an effort to improve ABJ and conduct PSN regularly and thoroughly

Dengue Hemorrhagic Fever (DHF) risks causing death when the sufferer experiences shock due to bleeding. Until now, there is no specific drug to cure DHF. This study was aimed to determine the effect of interpersonal communication on improving the prevention of Dengue Fever (DHF). The type of research used is Quasi-experiment with nonrandomized pretest-posttest control group design. Samples were taken by simple random sampling of 78 respondents from a population of 1,791 households each of 39 respondents in the intervention group and 39 in the control group. Data collection uses questionnaires and interviews in the working area of the Baubau City Meo-Meo Community Health Center. Data were analyzed using the Wilcoxon and Man Whitney tests. The results showed the percentage of respondents characteristics in this study the most age groups were 28-38 years 39.74%, female sex 79.48%, married 92.31%, high school education 56.41%. The mean score increased knowledge (13.21 to 17.49), AND attitudes (33.38 to 38.92) about DHF before and after interpersonal communication. The mean score increased knowledge (12.69 to 16.49), and attitudes (33.33 to 37.46) about DHF before and after counseling. While the comparison of the intervention group was significant from the control group with (p <0.05) namely knowledge (p = 0,000), and attitude (p = 0.021) about DHF. Interpersonal communication is more influential than counseling and the need for monitoring and evaluating the implementation of interpersonal communication on an ongoing basis.

This study aimed to determine the effect of positive deviance approach on reducing the incidence of Dengue Hemorrhagic Fever (DHF) in Pangkep Regency. This studi used mix-method design to identify positive deviance behavior in preventing DHF and intervene by applying the positive deviance to the community in Pangkep Regency. The number of samples for quantitative data was 126 respondents and qualitative data was 12 informants. Data collection was conducted through Focus Group Discussion (FGD) and interviews. The research data were analyzed using logistic regression test and McNemar tests. The results showed the most influential factor in DHF incidence was positive deviance. Positive deviance behavior to prevent DHF were used long-sleeved clothing, use oil, and planting medicinal plants in the yard. The results of the intervention showed there was a significant influence of intervention using a positive deviance approach to increasing the planting of medicinal plants in the yard to prevent DHF, and there was a decrease in the incidence rate of DHF in Pangkep Regency. Positive deviance approach could be used as an effort to prevent and control DHF.
This study aimed to determine the effect of positive deviance approach on reducing the incidence of Dengue Hemorrhagic Fever (DHF) in Pangkep Regency. This studi used mix-method design to identify positive deviance behavior in preventing DHF and intervene by applying the positive deviance to the community in Pangkep Regency. The number of samples for quantitative data was 126 respondents and qualitative data was 12 informants. Data collection was conducted through Focus Group Discussion (FGD) and interviews. The research data were analyzed using logistic regression test and McNemar tests. The results showed the most influential factor in DHF incidence was positive deviance. Positive deviance behavior to prevent DHF were used long-sleeved clothing, use oil, and planting medicinal plants in the yard. The results of the intervention showed there was a significant influence of intervention using a positive deviance approach to increasing the planting of medicinal plants in the yard to prevent DHF, and there was a decrease in the incidence rate of DHF in Pangkep Regency. Positive deviance approach could be used as an effort to prevent and control DHF.

Introduction: Estimating the incidence of Dengue Hemorrhagic Fever (DHF) in the future is critical to anticipate the incidence of DHF. This study aims to estimate dengue fever incidence in Makassar City in the next 20 years (2020-2040) using a dynamic systems approach. Methods: This study uses the Research and Development (R&D) method with a dynamics system approach in Makassar City. The sample in this study is data on dengue cases in Makassar City from 2012 to 2016. The right policy scenario in reducing dengue cases is carried out using Interpretative Structural Modeling (ISM). The PowerSim program is used to analyze the dynamic model of DHF incidence. Results: The results showed that the critical elements of DHF prevention were jumantik, 3M Plus, early warning systems, and counseling. Based on a dynamic model simulation for 20 years in Makassar City by applying the jumantik scenario, 3M Plus, early warning systems, counseling, and the combined procedure, the estimated average dengue incidence has decreased by 2.3%, 27.7%, 52.2%, 11.71, respectively. % and 95.7%. Conclusion: The incidence of dengue fever in Makassar City can be controlled by combining the jumantik program, 3M Plus, early warning systems, and counseling.