Online ISSN: 2515-8260

Keywords : parotid gland


HISTOLOGICAL AND HISTOCHEMICAL FINDING OF MAJOR SALIVARY GLANDS IN DOMESTIC GUINEA PIG

Iman Mousa Khaleel, Eyhab R. Al-Samawy , Mahdi Abdul kreem Atiea ,Hawraa faisal Mshal, Mustafa Salah Hasan

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 4, Pages 1947-1959

The current study aimed to determined the histological and histochemical structure of major salivary glands taken from six mature female and male guinea pig captured locally in Al-Samawa city (Province of Al-Muthanna, Iraq) after euthanized of animals, the major salivary glands fixed in 10 % NBF, dehydrated in series of graded concentrations of ethanol, clearing in xylol, embedded in paraffin. Sections of (5 μm) thick then stained with Mayer’s haematoxylin and eosin, PAS, combind PAS-AB (pH 2.5) and the Masson’s Trichrome. The results showed that the parotid gland was enveloped by thick dense fibrous connective tissue capsule and lumina of the acini are narrow.
Around the acinar cells, myoepithelial cells may be seen, and the intercal ated ducts are lined by simple cuboid epithelial cells. Submandibular glands were coated by a capsule of dense fibrous connective tissue from which septa of connective tissue extended and divide the gland into several lobules and sublingual glands which is compound tubuloacinar gland the paranchyma are lobules, enclosed by layer of irregular connective tissue capsule, from it, the septa are raised and dividing it into various lobules in conclusion the salivary gland’s of guinea pig capture in Al-Samawa showed some differences of histological structures.

Total conservative parotidectomy for management of benign parotid neoplasms

Dr. Apurva Agarwal, Dr. Manas Ranjan Deo, Dr. Santsevi Prasad, Dr. Sunil Kumar

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 1422-1426

Introduction: Salivary gland tumours contribute for about 3 - 10% of all neoplasms of the
head and neck; from this, benign tumours are commoner than their malignant counterpart.
The most common benign neoplasm of salivary glands is pleomorphic adenoma which
constitute about 60 – 70% incidence in the general population. The overall incidence of
salivary gland tumours is roughly remains unchanged throughout the world and there is no
significant predilection for either sex. In the early 1940s, intracapsular enucleation was
performed as the management for pleomorphic adenoma. Leaving the tumour capsule in-situ
resulted with 45% of its recurrence. Patey and Thackray explained that the capsule of the
tumour is often incomplete and therefore, a lumpectomy was suggested to be replaced by
other procedures available. Extra capsular dissection removes 2–3 mm border of healthy
tissues without damaging the facial nerve and partial superficial parotidectomy removes 2 cm
of normal parotid tissue with partial facial nerve dissection. Furthermore, SP versus TCP
carries the advantages of avoiding post-operative temporary facial nerve weakness and Frey’s
syndrome. Hence, there is also evidence that 60% of parotid tumours lie in close contact with
facial nerve and exposure of the tumour capsule remains a great concern.3 this retrospective
study assesses the immediate and long-term results of Total Conservative Parotidectomy in
patients with benign parotid neoplasms.

Curative Effect of Alpha Lipoic Acidon Parotid Gland Histopathological Changes in Adult Male Rats with Experimentally Induced Hypothyroidism

Marwa Mahmoud Ahmed, Manal M. S. El - Meligy, Norhan Nasser Mohamed, Nancy Hussieny Hassan

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 3959-3970

Background:One of the most frequent chronic disorders is hypothyroidism. It affects various
organs including the salivary glands. It causes pathophysiological changes including
xerostomia. Alpha lipoic acid (ALA) can provide health benefits through its role as a potent
antioxidant, metal chelator and reducing agent of the oxidized forms of other antioxidants
such as vitamins C and E. Aim: To estimate the influence of hypothyroidism on rat’s parotid
gland and to illuminate the possible curative role of alpha lipoic acid(ALA). Methodology:
Thirty-six adult male rats were haphazardlyarranged into four groups; nine animals in each
as follows: Group 1 (control group): 4 rats were givenpurified water (vehicle of carbimazole)
for threeweeks, the other 5 rats were given a daily oral dose of 2ml /kg corn oil only (vehicle of
ALA) for four weeks after initiation of hypothyroidism. Group 2 (ALA group): were given a
daily oral dose of 60 mg /kg ALA dissolved in its vehicle for 4 weeks. Group 3 (hypothyroidinduced
group): were givena daily oral dose of 1.35 mg/kg of carbimazole liquified in its
vehicle for 3 weeks to induce hypothyroidism. Group 4 (hypothyroid- ALA group): were givena
daily oral dose of 1.35 mg/kg of carbimazole for 3 weeks followed by a daily oral dose of 60 mg
/kg ALA treatment for the consecutive 4 weeks.The animals were anaesthetized and
slaughtered at the end of the experiment. Parotid glands were collected, and the specimens
were prepared for light microscopic examination using Hematoxylin and Eosin and Masson's
trichrome stains were used, Morphometric analysis using ANOVA and Tukey's post hoc
testswas performed.Results: Hypothyroidism caused histopathological changes in the form of
general cellular distortion, cytoplasmic vacuolations, cellular infiltration, blood vessels
congestion and fibrosis and morphometric changes in the form of a significant increase of
collagen fibres in hypothyroid group. Alpha lipoic acid succeeded to improve those changes.
Conclusion: Hypothyroidism has destructive effects on the Parotid gland structure.
Fortunately, it is recommended to take Alpha Lipoic Acid in hypothyroid cases to help in
decreasingsuch effects.