Online ISSN: 2515-8260

Keywords : circANKRD36

The Expression Profile of Circular ANKRD36 as a Genetic Biomarker for Type 2 Diabetes Mellitus and Related Cardiometabolic Risk Factors

Nearmeen M. Rashad , Gehan A. Ebrahem, M.N. Ayman Abd-Elrahman, Marwa A.E. Ahmed

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 3106-3115

Background:Circular RNAs (circRNAs) are a class of non-coding RNAs (ncRNAs)
with covalently closed single-stranded structures. The epigenetic regulatory role of
ncRNAs in diabetes-associated cardiometabolic diseases is still evolving. This study
aimed to investigatewhether therelative expression level ofcirc. ankyrin repeat
domain36 (circANKRD36) are altered in Egyptian patients with T2DM and whether
they correlatedwith othercardiometabolic risk factors.
Methods: case-controlled study was conducted on forty patients with T2DM, and 20
healthy subjects were included in the study. Diagnosis of T2DM was based on ADA
Criteria.The expression profile of circANKRD36 was measured using quantitative realtime
(qRT) PCR.
Results: The relative expression levels of circANKRD36wassignificantly increased in
patients with T2DM compared to control. p˂0.001*.The relative expression levels of
circANKRD36 were significantly positive correlated with cardiometabolic risk
factorsand linear regression test revealed that hba1c as well as BMI, were
independently correlated with epigenetic markers. Our results revealed that the
sensitivities and the specificities ofcircANKRD36 expression levels to diagnosis of
T2DM were 87% and .99.15.
Conclusion: the relative expression levels of circANKRD36were significantlyincreased
in patients with T2DM.Also,the relative expression levels of circANKRD36 were
significantly positive correlated to cardiometabolic risk factors.