Online ISSN: 2515-8260

Keywords : Double immunostaining

Double Immunohistochemical Expression of FAK and PCNA in Odontogenic Keratocyst, Orthokeratinized Odontogenic Cyst, Dentigerous Cyst and Glandular Odontogenic Cyst

Seham Hazem El-Ayouti, Heba Mahmoud Dahmoush, Hatem Wael Amer

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 3335-3345

Background: Odontogenic cysts are distinctive disorders that affect oral and maxillofacial
tissues.The majority of odontogenic cysts such as dentigerous cysts and orthokeratinized
odontogenic cyst show an indolent course, whilst others demonstrate an aggressive behavior
and may have higher recurrence rate like odontogenic keratocysts and glandular odontogenic
cysts.Here wecompared FAK and PCNA expression in the previously mentioned cysts and
attempted to correlate the findings to their variable clinical behaviors.
Aim of the study:This study aimed to clarify the role of epithelial to mesenchymal transition in
invasion potential, proliferation and aggressiveness of these lesions, which had been achieved.
Methods: Double Immunostaining technique for FAK and PCNA was performed on the same
tissue specimens using two types of chromogens. DAB chromogen was used for the assessment
of nuclear expression of PCNA, while AEC chromogen was used for the assessment of
cytoplasmic expression of FAK.
Results: The greatest mean of the nuclear immunoexpression of PCNA was recorded in
glandular odontogenic cyst group, followed by the odontogenic keratocyst, while the greatest
mean area percent of the cytoplasmic expression of FAK was recorded in odontogenic
keratocyst group, followed by the glandular odontogeniccyst

FAK and PCNA Double Immunohistochemical Expression among Different Oral Squamous Cell Carcinoma Grades

Hatem Wael Amer, Sally Ibrahim Mohamed

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 2795-2805

Focal adhesion kinase (FAK) is a tyrosine kinase that belongs to the focal adhesion complex that proved to
have a serious role in cancer progression through its upregulation, while proliferating cell nuclear antigen
(PCNA) is a proliferation indicating marker that involved in tumors behavior regarding invasiveness. Our
aim is to assess the synchronization in the upregualtion of both markers through double immunostaining
and their correlation with prognosis worsening in well differentiated and poorly differentiated oral squamous
cell carcinoma (OSCC).
Study design:
A total number of 60 OSCC lesions distributed equally (n= 30) between the first group (well differentiated
OSCC) & second group of (poorly differentiated OSCC). Double immunostaining with FAK &PCNA was
carried out to determine their localization. Area percent measurement of FAK and nuclear count for PCNA
were performed and analyzed.
Both area percent measurement of FAK and nuclear count of PCNA are higher in the poorly differentiated
OSCC group than that of the well differentiated OSCC group, results showed statistically significant
difference between the groups.
Our results indicate that both FAK and PCNA are upregulated in poorly differentiated OSCC than in the
well differentiated OSCC supporting their role in cancer aggressiveness & progression together with poor
prognosis in higher grades of same tumor