European Journal of Molecular & Clinical Medicine

In this study, 60 blood samples were collected from pregnant and aborted women infected with Toxoplasmosis, while 30 blood samples were collected from non-infected women. 5 ml of venous blood was collected and placed in SST (Serum Separating Tube) tubes for 10-15 minutes to separate the serum before centrifuged for 10 minutes at 3500 rpm. FSH and LH concentrations, as well as IL-12 and IFN-γ kinetics, were measured using ELISA. The study's findings revealed a rise in the concentrations of IL-12 and IFN-γ, which were (10.17±0.20 pg/ml) and (8.67±0.18 pg/ml) in the affected women, respectively. It was also discovered that the concentration of IL-12 did not change between the two age groups (20-30) and (40-31) for female patients, where it reached (10.15±0.25) and (10.32±0.33) pg/ml, respectively, whereas the high concentration of  IFN-γ was recorded within the age group (40-31) for female patients, which amounted to (9.61 ±0.51pg/ml) compared to its concentration in the age group (20-30) for female patients, whichamounted to (8.40±0.14 pg/ml). The infected women likewise had high LH concentrations and low FSH concentrations, which were (14.85± 0.94)) and (5.20±0.607) mlU/ml, respectively. There was no difference in the concentration of LH hormone for female patients in the two age groups (20-30) and (40-31), as it was (15.08± 1.28) (14.21 0.28) mlU/ml respectively., and no difference was shown in the concentration of FSH among the female patients in the age groups (20-30) and (40-31), its concentration was (5.58 ± 0.90) and (4.11 ± 0.13) mlU/ml respectively.

The research dealt with the study of secondary amenorrhea and secondary amenorrhea and its relationship to the physiological and hormonal status of women, which is one of the gynecological diseases with serious effects on women with this disease, because cardiovascular diseases, osteoporosis, diabetes, infertility and mental state disorder are among the most important complications of this disease. . The study was conducted on (40) serum samples from Secondary Amenorrhea patients after their condition was diagnosed through clinical and laboratory tests. The ages of the patients ranged between (12-42) years. The levels of the hormones LH, FSH, E2, and Prog were measured, and the results were the presence of high significant differences (P <0.05) when measuring the level of hormones LH, FSH, E2, and Prog in the blood serum of the patients with secondary menopause and compared them with their concentrations in the control group. There were high significant differences (P <0.05) in serum levels of LH, FSH, and E2 hormones. The presence of high levels of the hormone LH, FSH, in the serum of most patients. The presence of a decrease in the levels of the hormones E2 and Prog in the serum of the majority of patients

Background: PCOS is the most common endocrinological disorder affecting 6 -14% amongst reproductive age group. The pathophysiology of PCOS is complex and it is still not well understood. Many markers have been studied to correlate between homocysteine level and other clinical, metabolic and hormonal profile. Preliminary investigations indicate that in women with PCOS, serum biomarkers of cardiovascular disorders such as homocysteine are abnormal. Furthermore the interactions between homocysteine and PCOS, biochemical features such as obesity, insulin resistance and higher levels of androgen, have been researched and the potential determinant of this finding is still being explored.
Aim and Objectives: We aim to determine association between hyperhomocysteinemia and PCOS. The objectives of this study is to evaluate and compare clinical, metabolic and 
hormonal profile in women of PCOS with normal homocysteine level with that of increased homocysteine level.
Materials and Methods: This is a prospective observational study which will be performed in the department of obstetrics and gynaecology, AVBRH, DMIMS (Deemed to be University, Wardha), a tertiary care teaching hospital situated in the rural area of Wardha district in over 140 patients for period between 2020-22. This study will include PCOS patients based on Rotterdam diagnostic criteria and will exclude patients with known metabolic, cardiovascular and endocrine disorders. Metabolic and hormonal profile will be evaluated with BMI, Lipid profile, Insulin resistance, serum levels of LH, FSH, DHEA and Testosterone in all patients. Serum homocysteine levels will be measured using ELISA.
Expected Results: We expect that there will be significant difference in clinical, metabolic and hormonal profile of PCOS women having hyperhomocysteinemia than women having normal homocysteine level.