Online ISSN: 2515-8260

Keywords : pseudomonas


Change in findings of culture and sensitivity data if any in chronic otitis media during COVID pandemic

Raghvendra Singh Gaur, Shammi Kumar Jain, Sanjay Agrawal, Dr. Swati Chandel

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 2, Pages 1067-1073

Background: Chronic otitis media is an important cause of morbidity affecting all ages leading to variety of complications which has major impact on the patients and more so in Covid pandemic. Study to see the change in findings of culture and sensitivity data in chronic Otitis media during Covid pandemic and hence modify the treatment plan.
Aims: To study the bacteriology associated with the chronic otitis media and study any change in findings of bacteriology of chronic otitis media during COVID pandemic.
Subjects and methods: The retrospective study was done. The culture and sensitivity data during Covid pandemic was collected from Jan 2020 to Jan 2021 from ENT centre and their analysis and interpretation done according to age, types of culture and their sensitivity.
Result: In the total 100 cases of culture and sensitivity results during pandemic, pseudomonas aeruginosa was the most common organism isolated and showed highest sensitivity towards piperacillin/tazobactam and polymyxin B. Staphylococcus aureus was second most common which showed highest sensitivity towards linezolid and teicoplanin. Both showed lowest sensitivity towards ciprofloxacin. There was no change found in the bacteriology culture during the Covid pandemic which was compared from available literature.
Conclusion: the change in the findings of culture and study of chronic otitis media based on the study done shows almost no difference with the pre-pandemic times. But high antibiotic resistance implicates the careful use of antibiotics in treatment and avoiding empirical method of treatment.

VIRULENCE MARKERS ANDMOTILITY PATTERNS EXHIBITED BY PSEUDOMONAS SPP. ISOLATED FROM HIV AND NON-HIV POPULATION WITH LRTI.

Anitha C; Senthamarai S; Sivasankari S

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 1002-1014
DOI: 10.31838/ejmcm.07.09.101

Detection of biofilm, slime and motility patterns in Pseudomonas spp. can be valuable in empathetic the virulence of the creature. Here studies on the phenotypic methods of biofilm production, slime production and the motility patterns exhibited in 69 clinical isolates of Pseudomonas spp. and 2 environmental isolates were performed. Nineteen strains produced biofilm in which 6 were from HIV and 13 were from Non-HIV patients. All the 19 isolates positive for biofilm production were taken for detection of biofilm genes by PCR. The gene coding for alkaline protease aprA gene was detected in 13 (68.42%) isolates. The gene for phenazine biosynthesis phzA1 gene was detected in 14 isolates. The house keeping gene acpP was found to be positive for 18 (94.7%) isolates. 48 isolates were found positive for slime production. Pellicle formation was positive in 39 isolates. Swarming motility was positive in 42 isolates 29 were from HIV and 11 were from Non-HIV. Swimming motility was exhibited in 31 isolates. Twitching motility was exhibited in 35 isolates. The 2 environmental isolates were found to be positive only for slime production, swarming, and swimming motility. Among the clinical isolates, HIV isolates showed high production of slime, pellicle and positivity for the motility patterns seemed to be high in HIV compared to Non-HIV isolates except for the biofilm production which is high in Non-HIV isolates.