Online ISSN: 2515-8260

Author : Amer, Seham Hazem El-Ayouti, Heba Mahmoud Dahmoush, Hatem Wael

Double Immunohistochemical Expression of FAK and PCNA in Odontogenic Keratocyst, Orthokeratinized Odontogenic Cyst, Dentigerous Cyst and Glandular Odontogenic Cyst

Seham Hazem El-Ayouti, Heba Mahmoud Dahmoush, Hatem Wael Amer

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 3335-3345

Background: Odontogenic cysts are distinctive disorders that affect oral and maxillofacial
tissues.The majority of odontogenic cysts such as dentigerous cysts and orthokeratinized
odontogenic cyst show an indolent course, whilst others demonstrate an aggressive behavior
and may have higher recurrence rate like odontogenic keratocysts and glandular odontogenic
cysts.Here wecompared FAK and PCNA expression in the previously mentioned cysts and
attempted to correlate the findings to their variable clinical behaviors.
Aim of the study:This study aimed to clarify the role of epithelial to mesenchymal transition in
invasion potential, proliferation and aggressiveness of these lesions, which had been achieved.
Methods: Double Immunostaining technique for FAK and PCNA was performed on the same
tissue specimens using two types of chromogens. DAB chromogen was used for the assessment
of nuclear expression of PCNA, while AEC chromogen was used for the assessment of
cytoplasmic expression of FAK.
Results: The greatest mean of the nuclear immunoexpression of PCNA was recorded in
glandular odontogenic cyst group, followed by the odontogenic keratocyst, while the greatest
mean area percent of the cytoplasmic expression of FAK was recorded in odontogenic
keratocyst group, followed by the glandular odontogeniccyst