Online ISSN: 2515-8260

Author : Arunachalam, Pushparaj

Proteomic approaches reveal the deregulation of Apoptotic proteins in MCF7 breast cancer cell line treated byred seaweed, Gracilaria corticota

Ananthi Sivagnanam; Balasankar Thangasamy; Ambika Pushparaj; Pushparaj Arunachalam

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 2982-2987

Background: Breast cancer is always known to be a major health problem globally. Though
many advancements are achieved in treatment strategy for breast cancer, still the patient survival
rate is not promising for all the affected patients. Hence there is an emerging need for an
alternative medicine for treating cancer patients.
Objective: Thus, we aimed to understand the possibility of using commonly available red
seaweed, Gracilaria corticota in treating breast cancer. To analyse this, we aimed to check the
seaweed extract’s efficiency in breast cancer cell lines, MCF 7 using proteome wide analysis.
Method: The MCF7 cells were treated with red seaweed extract and incubated for 48 hrs.
Further the treated cells were scraped and lysed to extract the proteins. The extracted proteins
were estimated and further subjected to proteomic profiling. To analyse the differential protein
expression and identification of the proteins, two-dimensional electrophoresis coupled with mass
spectrometry was adapted.
Results: We found upon seaweed extract treatment in MCF7 breast cancer cell line, important
apoptotic proteins were deregulated. These proteins include the down regulation of
BCL2,BCL2L1 and MCL1 and upregulation of BAX protein upon treatment when compared to
untreated cells.

Significance of Serum Samples for Protein Studies

Pushpalakshmi Gangadharan; Ananthi Sivagnanam; Balasankar Thangasamy; Ambika Pushparaj; Premkumar Karunakaran; Raja JeyaSekar R; Megala Jayaraman; Pushparaj Arunachalam

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 740-749

Serum proteins also known as blood or plasma proteins are proteins present in
blood that serve many different functions, including transport of lipids, hormones,
vitamins, and minerals in the circulatory system and the regulation of acellular activity
and functioning of the immune system. Other blood proteins act as enzymes, complement
components, protease inhibitors, or kinin precursors. Although serum proteins have very
high concentrations, they exhibit an uneven distribution in terms of composition. That is,
only about 22 proteins account for 99% of all the serum proteins. These include serum
albumin, globulins, and fibrinogen. The remainder 1% of blood proteins is composed of
low abundance circulatory proteins, as well as proteins secreted by live, apoptotic, and
necrotic cells. Most blood proteins are secreted by the liver and intestines except for the
gamma globulins, which are synthesized by the immune system. In this study we aimed to
analyse the serum samples taken from gastric cancer patients and compared with healthy
serum samples. We estimated the serum samples using Bradford method of protein
estimation and performed SDS PAGE analysis. This article highlights the importance of
serum sample collection, handling and storage with respect to consideration towards
protein-based studies using serum samples.

Significance of Complement factor and Immunoglobulin in Helicobacter pylori induced Gastric Cancer

PushpalakshmiGangadharan .; AnanthiSivagnanam .; SubekshaGovindaRajan Ravi; Chaitanya Vattem; Premkumar Karunakaran; Raja JeyaSekar R; Megala Jayaraman; Pushparaj Arunachalam

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 128-142

Background: Gastric cancer (GC) still found to be the predominant cancer in Asia.There were very few reports which were focused on population specific biomarker studies with respect to Helicobacter pylori (H. pylori) induced gastric cancer in India. Hence, we aimed to perform this biomarker study involving H. pylori positive and negative healthy serum samples in comparison with H. pylori positive gastric cancer samples.
Methods: We have adapted Proteome based approaches to identify serum biomarkers in our Indian population samples. For proteins separation and identification, 2D gel electrophoresis with tandem mass spectrometry approach was performed and thirty differentially regulated protein spots were analyzed.