Information from ethnic groups or indigenous traditional medicine hasplayed a vital role in the discovery of novel products from plants as chemotherapeuticagents. Wound healing is a complex and complicated process. It runs through a number of phases, which either run concurrently or are intimately interlinked through some chemical, biochemical and cellular pathways.Juglans regia is a medicinal plant which is widely used for the treatment of many ailments.The array of human health benefits derived from this plant is due to the abundant presence of the different phytochemical compounds present in it. Plant material (leaves) of Juglans regia of family Juglandaceae was collected from Saloora Ganderbal, Kashmir. The preliminary phytochemical screening of the plant material was performed which showed the presence of many phytochemical compounds in it. The extracts were formulated 1% w/v in gum acacia dissolved in normal saline. JR (Juglans regia) 1% w/v was used as dose. A full thickness of the excision wound (400mm2appx.) was created along the markings using toothed forceps, a surgical blade and pointed scissors.It was observed that wound contractingability of animals treated with ointment containing 1% (w/w)methanolic extracts of Juglans regia were found to be significantly higher (P < 0.001)on days 8th, 12th and 16th as compared to the control group.