Background: Vascular Endothelial Growth Factor –A (VEGF-A) has a vital role in angiogenesis inhematological malignancies including acute lymphoblastic leukemia (ALL).Many singlenucleotide polymorphisms(SNPs) in VEGF gene may affect susceptibility of ALL in children. Aim of the study: To investigate the frequency of -460 VEGF gene SNP and their impact on plasma level of VEGF-Ain children with ALL. Material and method: This study included 40 children with ALL and 40 healthy children as controlcarriedout at the Oncology Unit in Basrah Children specialty Hospital from Oct 2019 to March 2020. VEGF-A plasma level evaluated using ELISA assay, PCRteqnique was used to detect the -460 VEGF SNP. Result:VEGF-Aplasma level in ALLchildren was higherthan control, there is significant difference of -460 VEGFSNP between ALLchildren and healthy control and the impact of gene polymorphism on plasma level of VEGF-A in ALL children was highly significant. Conclusion: VEGF-A level and gene SNPcould play a central role in ALL pathogenesisand progression.