Abstract : Background:- the aim of the present study was to evaluate component of variety color of gallstone samples using Fourier Transform Infrared Spectroscopic Technique (FTIR) , also study the cost and simple method of Fourier Transform Infrared Spectroscopic Technique (FTIR) compare with result of quantitative chemical analysis like total cholesterol, calcium , total bilirubin , total protein . Material and method:- the material in the study were gallstones removed from 140 patient ( 94 female, 46 male) after surgical. All samples were stored in sterile dried counter and used for FTIR spectral analysis and quantitative chemical analysis. Result :-the results of FTIR were suggested that cholesterol and mixed gallstones content were a major component , cholesterol gallstones were characterized by the band 2949,1456,1053 cm -1 , while in mixed stones the band of cholesterol between 2800-3000 cm1 due to asymmetrical stretch vibration of CH2 and CH3group, and quantitative chemical analysis were explained the cholesterol stones and mixed stones were higher cholesterol compared to pigmented stones which were richer in total bilirubin and total protein. Conclusion:-the investigation were suggested thatFourier Transform Infrared Spectroscopic Technique (FTIR) is the less cost and simple method to find component of differential gallstone samples and this method conformed the cholesterol and mixed with calcium carbonate , bilirubin and total protein is the major component of human gallstones from Babylon,Iraq.