Aims:To evaluate the bacteriological profile and antibiotic sensitivity pattern of isolates from blood culture. Methods: In all, 610 patients with a suspected blood stream infection were recruited for this research from the different OPDs, IPDs, and ICUs located within the hospital. Standard microbiological procedures, including colony character, gram staining, motility testing, and standard biochemical tests, were used throughout the process of isolating and identifying the organisms. Antimicrobial susceptibility test Kirby Bauer's disc diffusion technique was used on Mueller Hinton agar (MHA) in accordance with the recommendations provided by the CLSI to test antimicrobial sensitivity.