Study of the health and medical technology student’s knowledge and attitudes will open the ways to solve the deficit in their information regarding radiation hazards. The aim is to assess the knowledge of Al-Zahra university students toward radiation hazard.This study is a cross-sectional observational analytical study of the amount of knowledge and altitude towards radiation hazards and protection of the Health and Medical Technology students’ of Al-Zahra University in Karbala, Iraq. This cross-sectional questionnaire-based study was conducted on 129 out of 132 undergraduate students from The Health and Medical Technology College, whose curriculum included General Radiology, after completing 90 days in the Department of Radiological Techniques at Al-Zahra University for Women. Among 132 students, 129 students participated in this Google Classroom questionnaire, giving an overall response rate of (97.7%). Their overall knowledge was good and showed a higher KAP value in relation to hazardous protection that (Is x ray is harmful) with a percentage of (66.6%), while their knowledge was poor concerning the safety guidelines. The lack of knowledge about ALARA or ALADA principles needs to be considered by updating first year student’s curriculum as well as creating training courses to improve their knowledge.