This study attempts to analyze the differences of knowledge and attitudes of construction labours of Engineering Faculty Unhas, Gowa with methods of communicating information and education as well as the screening. In response to the prevention of HIV/AIDS at work. This study using the method was an experiment quasi pre and post-test design with the application of two group pre and post. With different treatment given a few and observed every treatment. This study found that there is the influence of intervention with the IEC media methods before and after interventions affecting knowledge and attitude labourers of HIV/AIDS, and some the influence of intervention with the method the screening before and after interventions affecting knowledge and attitudes labourers about HIV/AIDS. Based on our research findings, there need to be continuous interventions to increase knowledge, much efforts to prevent sexually transmitted infection and HIV/AIDS and check periodically of HIV/AIDS.