Background and objectives: Rotaviruses are the major cause of gastroenteritis in infants and young children worldwide. In India the mortality and economic burden associated with rotavirus is high. The purpose of the present study was to know the prevalence of rotavirus diarrhea in children in and around Bihar. Material and methods: The study was conducted in Department of Microbiology, IGIMS, Patna. Study duration of Two years. The study included 120stool samples from pediatric patients of age group 6 months to 5 years. The samples were tested for the presence of rotavirus antigen using ELISA, (Enzyme linked immunosorbent assay) latex agglutination (LA) and immunochromatography (ICG) test. Conclusion: Rotavirus infection was seen in a considerable proportion of infants and children in present study. Latex agglutination and immunochromatography test werefound to have good sensitivity and specificity, when compared with ELISA, for detection of rotavirus antigen. These tests can be used for screening cases of suspected rotavirus diarrhea during an epidemic outbreak.