Background: Oral health is traditionally defined as an oral status that is free of diseases, which not only makes people look beautiful, but also contributes to the normal function of mouth. Aim: To evaluate self-reported oral hygiene practice and knowledge in dental students of Narsinhbhai Dental College and Hospital, Visnagar. Method: This cross-sectional study was conducted on total 391 voluntarily participated students of Narsinhbhai Patel Dental College, in the month of October 2020. It included 151 students of pre-clinical group, 166 students of clinical group, 74 students in the group of interns. A total of 15 questions were designed to evaluate the oral hygiene practice and knowledge, in the format of multiple choice questions.