The NS is a kidney disease caused by an immunological response. It's linked to T-cell dysfunction and a secondary B-cell condition that causes abnormalities in immunoglobulin scales. This systemic disruption of T cell activity causes the generation of humoral factors or lymphokines, which cause the glomerular basement membrane to become more permeable.This study aims to identify the link between tumour necrosis factor-alpha single nucleotide polymorphisms (TNF-SNP) (-863 C/A) and the development of NS and their impact on TNF levels in the blood and steroid medication responsiveness.A total of 65 NS patients (42 males and 23 females, with a mean age of 7.23 3.15 years) and 30 healthy controls were included in the current study. They sought treatment at Al-Ima main Al-Kadima Medical City's Central Child Teaching Hospital in India's nephrology consultation clinic. Using a kit from Promega Company in India, DNA was extracted from whole blood samples according to the manufacturer's instructions. PCR-RFLP was used to find 863 C/A SNPs in the promoter of the TNF gene.In terms of age and sex, both cases and controls are comparable. Patients and controls had similar mean ages of 6.952.86 and 7.52 3.42 years (p = 0.516). Patients had 42 men and 23 females, while controls had 23 males and ten females (p = 0.624).This finding implies that, at least in confident NS children, illness activity is linked to TNF-serum levels. The lack of a drop in serum TNF- levels in patients with SRNS supports this hypothesis. Furthermore, the current findings show that the -863 SNP does not influence TNF- levels in the blood.