Background: According to WHO Global estimation in the year of 2014, shows that 10.6% of live baby births (in both cases normal vaginal delivery and caseation section) were preterm. It’s very important to control the neonatal mortality rates before it becomesa terrible problem for the country. The newborn period is the most vital because it’s a high-risk time to develop various health problem it may be related to genetically or it may be due to environmental causes. So, a mother or a health care provider must know about what are the warning signs for a baby may cause serious health problems. Objectives: To assess the level of knowledge among the post-natal mothers regarding neo-natal danger signs, in a view to developing an informational booklet. Methods: A non-experimental descriptive study with a quantitative survey research approach was conducted among 141 post-natal mothers by adopting a purposive sampling technique. Data was collected by the help of self-structure questionnaire tool through the interview technique. The informational booklet was given after the data was collected. Results: The study findings show that 48.9% of mothers having average knowledge regarding danger signs whereas 27.0% and 24.1% of mothers are having poor and good knowledge regarding danger signs respectively. It also shows that some of the socio-demographic variables like age, education of mothers and fathers, occupation of mother, family status, types of residence, parity status of mother and age of new-born are statistically significant with the level of knowledge keeping p value=0.05. Conclusion: The study concludes that overall mothers are having average knowledge regarding neonatal danger signs, they are the primary caregiver of a new-born so that they need to improve their knowledge by counselling or other informational methods like programme or booklets during their pregnancy or after delivery of the baby.